PXD036624

PXD036624 is an original dataset announced via ProteomeXchange.

Title	293T NME4 TAP-MS and TurboID-MS
Description	To identify interacting proteins of NME4 in human cell lines, we using tandem affinity purification followed by mass spectrometry (TAP-MS) in HEK293T cells. We established stable cell line which expressing NME4 gene fused with SFB triple tags (S tag-2x Flag tag-SBP tag) or TurboID. We isolated, combined, and affinity-purified the membrane and soluble fractions using TAP. We identified proteins associated with the bait in the isolated complexes using MS and searched the uniprot human database for these proteins.
HostingRepository	PRIDE
AnnounceDate	2023-10-13
AnnouncementXML	Submission_2023-10-13_06:43:55.598.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	shaofangxie
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-12 03:54:42	ID requested
⏵ 1	2023-10-13 06:43:55	announced

Dataset with its publication pending

submitter keyword: TAP-MS TurboID-MS

XuLi
contact affiliation	School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China ( lab head )
contact email	lixu@westlake.edu.cn
lab head
shaofangxie
contact affiliation	westlake university
contact email	xieshaofang@westlake.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD036624

PRIDE project URI

• PRIDE
  1. PXD036624
     1. Label: PRIDE project
     2. Name: 293T NME4 TAP-MS and TurboID-MS