PXD036621

PXD036621 is an original dataset announced via ProteomeXchange.

Title	A methylotrophic bacterium growing with the antidiabetic drug metformin as its sole carbon, nitrogen and energy source
Description	Metformin is one of the most prescribed antidiabetic agents worldwide, and also considered for other therapeutic applications including cancer and endocrine disorders. Metformin is largely unmetabolised by human enzymes, and its mechanisms of action, likely also involving the microbiome, are not yet well characterised. Increasing presence of metformin in the environment has raised concerns, with reported toxic effects on aquatic life and potentially also on humans. We report on the isolation and characterisation of strain MD1, an aerobic methylotrophic bacterium growing with metformin as its sole carbon, nitrogen and energy source. Sequence analysis of its fully assembled genome allows its affiliation to Aminobacter niigataensis. Strain MD1 degrades metformin into dimethylamine used for growth, leaving guanylurea as a side-product. Differential proteomics and transcriptomics, as well as minitransposon mutagenesis of the strain, point to genes and proteins potentially associated with hydrolytic C-N cleavage of metformin, transport of metformin and guanylurea, and associated regulatory processes. Our results will inform future research on the fate of metformin and its degradation products in the human gut and the environment, and to identify microbial players and enzymes involved in the transformation of pharmaceuticals.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:00.713.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036621
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lauriane Kuhn
SpeciesList	scientific name: unclassified Aminobacter; NCBI TaxID: 2644704;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-12 03:53:35	ID requested
1	2023-03-11 05:22:48	announced
⏵ 2	2023-11-14 08:43:01	announced	2023-11-14: Updated project metadata.

Chaignaud P, Gruffaz C, Borreca A, Fouteau S, Kuhn L, Masbou J, Rouy Z, Hammann P, Imfeld G, Roche D, Vuilleumier S, A Methylotrophic Bacterium Growing with the Antidiabetic Drug Metformin as Its Sole Carbon, Nitrogen and Energy Source. Microorganisms, 10(11):(2022) [pubmed]

10.6019/PXD036621;

submitter keyword: dimethylamine, functional genomics, methylotrophy,metformin, Aminobacter, genome analysis, minitransposon mutagenesis, guanylurea, micropollutants, biodegradation

Stéphane Vuilleumier
contact affiliation	Génétique Moléculaire, Génomique, Microbiologie, Université de Strasbourg, UMR 7156 CNRS, 67000 Strasbourg, France.
contact email	vuilleumier@unistra.fr
lab head
Lauriane Kuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036621

PRIDE project URI

• PRIDE
  1. PXD036621
     1. Label: PRIDE project
     2. Name: A methylotrophic bacterium growing with the antidiabetic drug metformin as its sole carbon, nitrogen and energy source