PXD036613

PXD036613 is an original dataset announced via ProteomeXchange.

Title	Identification of Endos interacting proteins in Drosophila embryos by immunoprecipitation and LC-MS-MS/MS analysis
Description	The transition from oocyte to embryo requires translation of maternally provided transcripts that in Drosophila is activated by Pan Gu kinase to release a rapid succession of 13 mitotic cycles. Mitotic entry is promoted by several protein kinases that include Greatwall/Mastl, whose endosulfine substrates antagonize Protein Phosphatase 2A (PP2A), facilitating mitotic Cdk1/Cyclin B kinase activity. Here we show that hyperactive greatwallScant can not only be suppressed by mutants in its Endos substrate, but also by mutants in Pan Gu kinase subunits. Conversely, mutants in me31B or trailer hitch (tral), which encode a complex that represses hundreds of maternal mRNAs, enhance greatwallScant. Me31B and Tral proteins, known substrates of Pan Gu kinase, copurify with Endos. This echoes findings that budding yeast Dhh1, orthologue of Me31B, associates with Igo1/2, orthologues of Endos and substrates of the Rim15 orthologue of Greatwall. endos-derived mutant embryos show reduced Me31B and elevated transcripts for the mitotic activators Cyclin B, Polo and Twine/Cdc25. Together, our findings demonstrate a previously unappreciated conservation of the Greatwall-endosulfine pathway in regulating translational repressors and its interactions with the Pan Gu kinase pathway to regulate translation and/or stability of maternal mRNAs upon egg activation.
HostingRepository	PRIDE
AnnounceDate	2024-08-09
AnnouncementXML	Submission_2024-08-09_05:08:23.458.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hélène Rangone
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-12 03:40:50	ID requested
⏵ 1	2024-08-09 05:08:24	announced

10.1098/rsob.240065;

Rangone H, Bond L, Weil TT, Glover DM, oocyte-to-embryo transition. Open Biol, 14(6):240065(2024) [pubmed]

submitter keyword: Endos, syncytial embryo, LC-MS-MS/MS, immunoprecipitation, Drosophila

David Moore Glover
contact affiliation	- University of Cambridge, Department of Genetics, Downing Street, Cambridge CB2 3EH, UK - Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd, Pasadena, California 91125, USA
contact email	dmglover@caltech.edu
lab head
Hélène Rangone
contact affiliation	University of Cambridge, Department of Genetics
contact email	hr257@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD036613

PRIDE project URI

• PRIDE
  1. PXD036613
     1. Label: PRIDE project
     2. Name: Identification of Endos interacting proteins in Drosophila embryos by immunoprecipitation and LC-MS-MS/MS analysis