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PXD036610

PXD036610 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein interactors of MEG-1 in C. elegans embryos
DescriptionIn animals with germ plasm, specification of the germline involves “germ granules”, cytoplasmic condensates that enrich maternal transcripts in the germline founder cells. In C. elegans embryos, P granules enrich maternal transcripts, but surprisingly P granules are not essential for germ cell fate specification. Here we have described a second condensate in the C. elegans germ plasm. Like canonical P-bodies found in somatic cells, “germline P-bodies” contain regulators of mRNA decapping and deadenylation and, in addition, the intrinsically-disordered proteins MEG-1 and MEG-2 and the TIS11-family RNA-binding protein POS-1. Embryos lacking meg-1 and meg-2 do not stabilize P-body components, miss-regulate POS-1 targets, miss-specify the germline founder cell, and do not develop a germline. Our findings suggest that specification of the germ line involves at least two distinct condensates that independently enrich and regulate maternal mRNAs in the germline founder cells.
HostingRepositoryPRIDE
AnnounceDate2022-10-05
AnnouncementXMLSubmission_2022-10-04_16:45:26.957.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMadeline Cassani
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListphosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-12 03:38:41ID requested
12022-10-04 16:45:27announced
Publication List
10.1242/DEV.200920;
Keyword List
submitter keyword: C. elegans, MEG-1, germ granules, p-bodies
Contact List
Geraldine Seydoux
contact affiliationJohns Hopkins University School of Medicine
contact emailgseydoux@jhmi.edu
lab head
Madeline Cassani
contact affiliationJohns Hopkins University
contact emailmcassan1@jh.edu
dataset submitter
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Dataset FTP location
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