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PXD036593

PXD036593 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn vitro characterization of caspase-3 cleavage sites by LATE and HYATNE
DescriptionProteins' N-termini contain information about their biochemical properties and functions, and they can undergo co- or post-translational modifications, as well as be processed by proteases. To expand the coverage of the N-terminome, we have developed LATE (LysN Amino Terminal Enrichment), a method that uses selective chemical derivatization of α-amines to isolate the N-terminal peptides. We applied LATE in combination with other N-terminomic method to study caspase-3 mediated proteolysis both in vitro and during apoptosis in cells. This enable us to identify many unreported caspase-3 cleavages, including some that cannot be identified by other methods. Furthermore, we find direct evidence that some of the caspase-3 generated neo-N-termini can be further modified by Nt-acetylation. This provides a global picture of the caspase-3 degradome and uncovers previously unrecognised functional crosstalk between post-translational Nt-acetylation and caspase proteolysis pathways.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:50:53.997.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOded Kleifeld
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonomethylated residue; acetylated residue; alpha-amino dimethylated residue; iodoacetamide derivatized residue; N6,N6-dimethyl-L-lysine
InstrumentQ Exactive HF; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-08 07:50:42ID requested
12024-10-07 13:47:27announced
22024-10-22 05:50:54announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.mcpro.2023.100584;
Hanna R, Rozenberg A, Saied L, Ben-Yosef D, Lavy T, Kleifeld O, In-Depth Characterization of Apoptosis N-Terminome Reveals a Link Between Caspase-3 Cleavage and Posttranslational N-Terminal Acetylation. Mol Cell Proteomics, 22(7):100584(2023) [pubmed]
Keyword List
submitter keyword: LATE, HYTANE,LysN, Peptidyl-Lys metalloendopeptidase N-terminomics, Caspase-3
Contact List
Oded Kleifeld
contact affiliationFaculty of Biology, Technion - Israel Institute of Technology Haifa, Israel
contact emailokleifeld@technion.ac.il
lab head
Oded Kleifeld
contact affiliationTechnion
contact emailokleifeld@technion.ac.il
dataset submitter
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Dataset FTP location
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