PXD036582 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The endothelial inflammatory repertoire: a multi-omic delineation of cytokine induced endothelial inflammatory states |
Description | Vascular endothelial cells (ECs) form a dynamic interface between blood and tissue and play a crucial role in the progression of vascular inflammation. Here, we explored the underlying molecular mechanisms of endothelial-cytokine responses which govern inflammation. Applying an unbiased cytokine library, we determined TNFα and IFNγ induced the largest EC response and had distinct proteomic inflammatory signatures. Moreover, combined TNFα + IFNγ stimulation induced a third synergetic inflammatory state. We employed a multi-omics approach combining (phospho-) proteome, transcriptome and secretome to delineate these inflammatory states and found that endothelial cells contain a wide-array of immune-modulating capacities such as complement proteins, MHCI and MHCII complexes and distinct secretory cytokine production per stimulus. Synergy was regulated through cooperative interplay of transcript induction. This extensive resource describes the intricate molecular mechanisms that are at the basis of endothelial inflammation and supports the adaptive immunomodulatory role of the endothelium in host defense and vascular inflammation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:48:10.017.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stijn Groten |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-08 07:07:46 | ID requested | |
1 | 2023-05-17 13:37:39 | announced | |
⏵ 2 | 2023-11-14 08:48:12 | announced | 2023-11-14: Updated project metadata. |
Publication List
Groten SA, Smit ER, Janssen EFJ, van den Eshof BL, van Alphen FPJ, van der Zwaan C, Meijer AB, Hoogendijk AJ, van den Biggelaar M, Multi-omics delineation of cytokine-induced endothelial inflammatory states. Commun Biol, 6(1):525(2023) [pubmed] |
Keyword List
submitter keyword: mult-omics, inflammation,Endothelial cells |
Contact List
Maartje van den Biggelaar, PhD |
contact affiliation | Department of Molecular Hematology, Sanquin Research, Amsterdam, the Netherlands |
contact email | m.vandenbiggelaar@sanquin.nl |
lab head | |
Stijn Groten |
contact affiliation | Sanquin Research |
contact email | s.groten@sanquin.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036582
- Label: PRIDE project
- Name: The endothelial inflammatory repertoire: a multi-omic delineation of cytokine induced endothelial inflammatory states