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PXD036582

PXD036582 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe endothelial inflammatory repertoire: a multi-omic delineation of cytokine induced endothelial inflammatory states
DescriptionVascular endothelial cells (ECs) form a dynamic interface between blood and tissue and play a crucial role in the progression of vascular inflammation. Here, we explored the underlying molecular mechanisms of endothelial-cytokine responses which govern inflammation. Applying an unbiased cytokine library, we determined TNFα and IFNγ induced the largest EC response and had distinct proteomic inflammatory signatures. Moreover, combined TNFα + IFNγ stimulation induced a third synergetic inflammatory state. We employed a multi-omics approach combining (phospho-) proteome, transcriptome and secretome to delineate these inflammatory states and found that endothelial cells contain a wide-array of immune-modulating capacities such as complement proteins, MHCI and MHCII complexes and distinct secretory cytokine production per stimulus. Synergy was regulated through cooperative interplay of transcript induction. This extensive resource describes the intricate molecular mechanisms that are at the basis of endothelial inflammation and supports the adaptive immunomodulatory role of the endothelium in host defense and vascular inflammation.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:48:10.017.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStijn Groten
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-08 07:07:46ID requested
12023-05-17 13:37:39announced
22023-11-14 08:48:12announced2023-11-14: Updated project metadata.
Publication List
Groten SA, Smit ER, Janssen EFJ, van den Eshof BL, van Alphen FPJ, van der Zwaan C, Meijer AB, Hoogendijk AJ, van den Biggelaar M, Multi-omics delineation of cytokine-induced endothelial inflammatory states. Commun Biol, 6(1):525(2023) [pubmed]
Keyword List
submitter keyword: mult-omics, inflammation,Endothelial cells
Contact List
Maartje van den Biggelaar, PhD
contact affiliationDepartment of Molecular Hematology, Sanquin Research, Amsterdam, the Netherlands
contact emailm.vandenbiggelaar@sanquin.nl
lab head
Stijn Groten
contact affiliationSanquin Research
contact emails.groten@sanquin.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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