PXD036581

PXD036581 is an original dataset announced via ProteomeXchange.

Title	Endothelial ICAM-1 adhesome recruits CD44 for optimal regulation of human cytotoxic T lymphocyte extravasation
Description	The endothelial lining of blood vessels is covered with a thin polysaccharide coat called the glycocalyx. This layer of polysaccharides contains hyaluronan that forms a protective coat on the endothelial surface. Upon inflammation, leukocytes leave the circulation and enter inflamed tissue by crossing inflamed endothelial cells, mediated by adhesion molecules such as ICAM-1/CD54. To what extent the glycocalyx participates in the regulation of leukocyte transmigration is not clear. During extravasation, leukocyte integrins cluster ICAM-1, resulting in the recruitment of a number of intracellular proteins and subsequent downstream effects in the endothelial cells. Using an unbiased proteomics approach, we identified the full ICAM-1 adhesome and identified 93 new subunits of the ICAM-1 adhesome. Interestingly, we found the glycoprotein CD44 as part of the glycocalyx to be recruited to clustered ICAM-1 specifically. Our data demonstrate that CD44 binds hyaluronan to the endothelial surface, where it locally concentrates and presents chemokines that are essential for leukocytes to cross the endothelial lining. Together, we discover a link between ICAM-1 clustering and hyaluronan-mediated chemokine presentation by recruiting hyaluronan to sites of leukocyte adhesion via CD44.
HostingRepository	PRIDE
AnnounceDate	2025-09-30
AnnouncementXML	Submission_2025-09-30_00:06:51.133.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Floris van Alphen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-08 07:00:29	ID requested
⏵ 1	2025-09-30 00:06:51	announced

van Steen ACI, Gr, ö, nloh MLB, Joosten S, van Alphen F, van den Biggelaar M, Nolte MA, Spaargaren M, van Buul JD, Schoppmeyer R, Endothelial ICAM-1 Adhesome Recruits CD44 for Optimal Transcellular Migration of Human CTLs. J Immunol, 211(3):377-388(2023) [pubmed]

10.4049/jimmunol.2200761;

submitter keyword: CD44,Cytotoxic T cell, inflammation, glycosaminoglycans, endothelial cells, transcellular, transendothelial migration, hyaluronic acid, hyaluronan, glycocalyx

Prof. Dr. J.D. van Buul
contact affiliation	Molecular Cell Biology Lab, Dept. Molecular Hematology Sanquin Research and Landsteiner Laboratory Academic Medical Center at the University of Amsterdam Plesmanlaan 125, 1066CX Amsterdam, the Netherlands Phone: +31205123042 E-mail: j.vanbuul@sanquin.nl
contact email	J.vanbuul@sanquin.nl
lab head
Floris van Alphen
contact affiliation	Sanquin Research
contact email	f.vanalphen@sanquin.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD036581

PRIDE project URI

• PRIDE
  1. PXD036581
     1. Label: PRIDE project
     2. Name: Endothelial ICAM-1 adhesome recruits CD44 for optimal regulation of human cytotoxic T lymphocyte extravasation