PXD036577

PXD036577 is an original dataset announced via ProteomeXchange.

Title	Mitotic spindle disassembly in human cells relies on CRIPT having hierarchical redox signals
Description	Swift and complete spindle disassembly in late mitosis is essential for cell survival, yet how it happens is largely unknown in mammalian cells. Here we used real-time live cell microscopy and biochemical assays to show that the primordial dwarfism (PD)-related cysteine-rich protein CRIPT dictates the spindle disassembly in a redox-dependent manner in human cells. This previously reported cytoplasmic protein was found to have a confined nuclear localization with a nucleolar concentration during interphase but was distributed to spindles and underwent redox modifications to form disulfide bonds in CXXC pairs during mitosis. Then, it directly interacted with, and might transfer a redox response to, tubulin subunits via a putative redox exchange among cysteine residues to induce microtubule depolymerization. Expression of CRIPT proteins with mutations of these cysteine residues blocked spindle disassembly, generating two cell types with long-lasting metaphase spindles or spindle remnants. Live-cell recordings of a disease-relevant mutant (CRIPTC3Y) revealed that microtubule depolymerization at spindle ends during anaphase and the entire spindle dissolution during telophase might share a common CRIPT-bearing redox-controlled mechanism.
HostingRepository	PRIDE
AnnounceDate	2022-09-12
AnnouncementXML	Submission_2022-09-12_09:05:48.595.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yushan Ding
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-08 06:58:30	ID requested
⏵ 1	2022-09-12 09:05:48	announced

Dataset with its publication pending

submitter keyword: CRIPT, Disassembly, Mitosis, Redox regulation, Spindle

Kehan Xu
contact affiliation	Beijing Bio-Tech Pack Technology Company Ltd.
contact email	support@biotech-pack.com
lab head
Yushan Ding
contact affiliation	Beijing Bio-Tech Pack Technology Company Ltd.
contact email	support@biotech-pack.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036577
     1. Label: PRIDE project
     2. Name: Mitotic spindle disassembly in human cells relies on CRIPT having hierarchical redox signals