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PXD036575

PXD036575 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAurora A phosphorylates PKM2 at T45.
DescriptionFlag-PKM2 was expressed in H1299 cells. Then the H1299 cells were treated with DMSO or 100nM MLN8237. Then, cell lysate was incubated with anti-Flag M2-agarose. Elutes were separated with 10% SDS-PAGE. The gel was stained with Coomassie Brilliant Blue. Visualized bands were excised and subjected to LC-MS/MS analysis. Then the phosphorylation sites of PKM2 were identified.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:42:49.699.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAoxing Cheng
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-08 06:56:27ID requested
12023-03-11 05:11:45announced
22023-11-14 08:42:50announced2023-11-14: Updated project metadata.
Publication List
Jiang Y, Wang T, Sheng D, Han C, Xu T, Zhang P, You W, Fan W, Zhang Z, Jin T, Duan X, Yuan X, Liu X, Zhang K, Ruan K, Shi J, Guo J, Cheng A, Yang Z, Aurora A-mediated pyruvate kinase M2 phosphorylation promotes biosynthesis with glycolytic metabolites and tumor cell cycle progression. J Biol Chem, 298(11):102561(2022) [pubmed]
Keyword List
submitter keyword: Aurora A
PKM2
Contact List
Zhenye yang
contact affiliationThe First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
contact emailzhenye@ustc.edu.cn
lab head
Aoxing Cheng
contact affiliationUniversity of Science and Technology of China
contact emailchengax@mail.ustc.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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