PXD036570

PXD036570 is an original dataset announced via ProteomeXchange.

Title	Stereoretentive Post-Translational Protein Editing
Description	Chemical post-translational methods now allow convergent side-chain editing of proteins as a form of direct chemical mutagenesis without needing to resort to genetic intervention. Current approaches that allow the creation of constitutionally native side-chains via C–C formation using off-protein carbon-centred C• radicals added to unnatural amino acid radical acceptor SOMOphile ‘tags’ such as dehydroalanine are benign and wide-ranging. However, they also typically create epimeric mixtures of D-/L-residues. Here we describe a light-mediated desulfurative method that, through the creation and reaction of stereoretained on-protein L-alanyl Cβ• radicals, allows Cβ–Hγ, Cβ–Oγ, Cβ–Seγ, Cβ–Bγ and Cβ–Cγ bond formation to flexibly generate site-selectively edited proteins with full retention of native stereochemistry under mild conditions from a natural amino acid. This methodology shows great potential to explore protein side-chain diversity and construct useful bioconjugates. This dataset contains LCMS data of tryptic digests of a PstS protein selectively modified by different radical acceptors (modification site D178). In total, 16 LCMS files are presented along with the results of the identification by MSFragger, corresponding to the variants of the PstS protein with different modifications.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:59.037.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nikita Levin
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-08 06:50:14	ID requested
1	2023-05-10 13:42:54	announced
⏵ 2	2023-11-14 08:46:59	announced	2023-11-14: Updated project metadata.

Fu XP, Yuan Y, Jha A, Levin N, Giltrap AM, Ren J, Mamalis D, Mohammed S, Davis BG, Stereoretentive Post-Translational Protein Editing. ACS Cent Sci, 9(3):405-416(2023) [pubmed]

submitter keyword: protein editing,PstS, chemical modification, stereoretentive, LCMS

Benjamin Guy Davis
contact affiliation	Rosalind Franklin Institute, Harwell Science and Innovation Campus, Oxford OX11 0FA, UK Department of Chemistry, University of Oxford, Oxford OX1 3TA, UK Department of Pharmacology, University of Oxford, Oxford OX1 3QT, UK
contact email	ben.davis@chem.ox.ac.uk
lab head
Nikita Levin
contact affiliation	Rosalind Franklin Institute
contact email	nikita.levin@rfi.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD036570

PRIDE project URI

• PRIDE
  1. PXD036570
     1. Label: PRIDE project
     2. Name: Stereoretentive Post-Translational Protein Editing