PXD036558 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Novel insights into redox-based mechanisms for auranofin- induced rapid cancer cell death |
Description | One of the significant features of cancer cells is a persistent pro-oxidative status. Therefore, compared to their normal counterparts, the malignant cells are generally more dependent on antioxidants for cell survival and more vulnerable to further oxidative insults via pharmacolog-ical interventions targeting cellular redox systems. This is the biological basis of oxidative stress- or redox-based anticancer strategies. Auranofin (AUF) is a promising repositioning anti-cancer molecule with a multifaceted mode of action that could be cancer cell type- or dose-dependent. Using triple-negative breast cancer cells, we evidenced that thioredoxin reduc-tase inhibition, the best-studied anticancer activity of AUF, is not sufficient to induce efficient cell death. Cytotoxic doses of AUF trigger rapid and global intracellular oxidative stress. Based on the indications from redox proteome data, we showed experimentally that AUF treatment triggered a dose-dependent S-phase arrest and disintegration of actin cytoskeleton structure. These findings on AUF-induced early effects should provide novel insights into the anticancer mechanisms of this promising molecule |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:25:25.052.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jean-Michel Camadro |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-08 06:35:49 | ID requested | |
1 | 2023-03-10 18:22:16 | announced | |
⏵ 2 | 2023-11-14 08:25:27 | announced | 2023-11-14: Updated project metadata. |
Publication List
Hatem E, El Banna N, Heneman-Masurel A, Ba, ï, lle D, Vernis L, Riquier S, Golinelli-Cohen MP, Guittet O, Valli, è, res C, Camadro JM, Qiu X, Hildebrandt N, Lepoivre M, Huang ME, Novel Insights into Redox-Based Mechanisms for Auranofin-Induced Rapid Cancer Cell Death. Cancers (Basel), 14(19):(2022) [pubmed] |
Keyword List
ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project |
submitter keyword: auranofin |
oxidative stress |
redox regulation |
redoxome |
cancer |
Contact List
Huang Meng-Er |
contact affiliation | Institut de Chimie des Substances Naturelles, CNRS, France |
contact email | meng-er.huang@cnrs.fr |
lab head | |
Jean-Michel Camadro |
contact affiliation | Institut Jacques Monod Université Paris Cité, CNRS |
contact email | jean-michel.camadro@ijm.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036558 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036558
- Label: PRIDE project
- Name: Novel insights into redox-based mechanisms for auranofin- induced rapid cancer cell death