PXD036535

PXD036535 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Integrated microRNA and secretome analysis of human endometrial organoid reveal the miR-3194-5p/Aquaporin/S100A9 module in regulating trophoblast functions
Description	Successful placentation requires delicate communication between endometrium and trophoblasts. The invasion and integration of trophoblasts into the endometrium during early pregnancy is crucial to placentation. Dysregulation of these functions is associated with various pregnancy complications such as miscarriage and preeclampsia. The endometrial microenvironment exerts an important influence on trophoblast cell functions. We hypothesized that the hormonal environment regulates the miRNA profile and secretome of the human endometrial gland, which subsequently modulates trophoblast functions during early pregnancy. By establishing the first secretome profiles and miRNA atlas of these endometrial organoids to the hormonal changes followed by trophoblast functional assays, we demonstrated that sex steroid hormones modulate aquaporins (AQP)1/9 and S100A9 secretions through miR-3194 activation in endometrial epithelial cells, which in turn enhanced trophoblast migration and invasion during early pregnancy.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:31.556.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036535
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jianlin Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-05 09:58:20	ID requested
1	2023-05-10 13:20:33	announced
⏵ 2	2023-11-14 08:46:32	announced	2023-11-14: Updated project metadata.

Publication List

10.6019/PXD036535;
Dong Y, Li J, Cao D, Zhong J, Liu X, Duan YG, Lee KF, Yeung WSB, Lee CL, Chiu PCN, Integrated MicroRNA and Secretome Analysis of Human Endometrial Organoids Reveal the miR-3194-5p/Aquaporin/S100A9 Module in Regulating Trophoblast Functions. Mol Cell Proteomics, 22(4):100526(2023) [pubmed]

10.6019/PXD036535;

Dong Y, Li J, Cao D, Zhong J, Liu X, Duan YG, Lee KF, Yeung WSB, Lee CL, Chiu PCN, Integrated MicroRNA and Secretome Analysis of Human Endometrial Organoids Reveal the miR-3194-5p/Aquaporin/S100A9 Module in Regulating Trophoblast Functions. Mol Cell Proteomics, 22(4):100526(2023) [pubmed]

Keyword List

submitter keyword: Secretome
Human endometrial organoid
iTRAQ
LC-MS/MS

submitter keyword: Secretome

Human endometrial organoid

iTRAQ

LC-MS/MS

Contact List

Philip C.N. Chiu
contact affiliation	The University of Hong Kong
contact email	pchiucn@hku.hk
lab head
Jianlin Li
contact affiliation	the University of Hong Kong
contact email	jannisli@connect.hku.hk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD036535

PRIDE project URI

Repository Record List

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