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PXD036527

PXD036527 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChemoproteomic profiling of O-GlcNAcylated proteins and identification of O-GlcNAc transferases in rice
DescriptionO-linked β-N-acetylglucosaminylation (O-GlcNAcylation) is a ubiquitous posttranslational modification occurring in both animals and plants. Thousands of proteins along with their O-GlcNAcylation sites have been identified in various animal systems, yet the O-GlcNAcylated proteomes in plants remain poorly understood. Here, we report a large-scale profiling of protein O-GlcNAcylation in a site-specific manner in rice. We first established the metabolic glycan labeling strategy (MGL) with N-azidoacetylgalactosamine (GalNAz) in rice seedlings, which enabled incorporation of azides as a bioorthogonal handle into O-GlcNAc. By conjugation of the azide-incorporated O-GlcNAc with alkyne-biotin containing a cleavable linker via click chemistry, O-GlcNAcylated proteins were selectively enriched for mass spectrometry (MS) analysis. A total of 1,591 unambiguous O-GlcNAcylation sites distributed on 709 O-GlcNAcylated proteins were identified. Additionally, 102 O-GlcNAcylated proteins were identified with their O-GlcNAcylation sites located within a serine/threonine-enriched peptide, causing ambiguous site assignment. The identified O-GlcNAcylated proteins are involved in multiple biological processes such as transcription, translation and plant hormone signaling. Furthermore, we discovered two O-GlcNAc transferases (OsOGTs) in rice. By expressing OsOGTs in Escherichia coli and Nicotiana benthamiana leaves, we confirmed their OGT enzymatic activities and used them to validate the identified rice O-GlcNAcylated proteins. Our dataset provides a valuable resource for studying O-GlcNAc biology in rice, and the MGL method should facilitate the identification of O-GlcNAcylated proteins in various plants.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:46:01.485.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCong Lei
SpeciesList scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationListcarbamoylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; complex glycosylation
InstrumentOrbitrap Fusion Lumos; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-05 09:47:35ID requested
12023-05-10 09:20:38announced
22023-11-14 08:46:02announced2023-11-14: Updated project metadata.
Publication List
Li X, Lei C, Song Q, Bai L, Cheng B, Qin K, Li X, Ma B, Wang B, Zhou W, Chen X, Li J, Chemoproteomic profiling of O-GlcNAcylated proteins and identification of O-GlcNAc transferases in rice. Plant Biotechnol J, 21(4):742-753(2023) [pubmed]
Keyword List
submitter keyword: O-GlcNAcylation, HCD pd EThcD, metabolic glycan labeling, plant glycobiology.,rice, chemoproteomic
Contact List
Jiayang Li
contact affiliationState Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China
contact emailjyli@genetics.ac.cn
lab head
Cong Lei
contact affiliationPeking University
contact emailconglei@pku.edu.cn
dataset submitter
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Dataset FTP location
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