PXD036518

PXD036518 is an original dataset announced via ProteomeXchange.

Title	Oilseed protein isolates as albumin alternatives for muscle cell culture media
Description	The development of cost-effective serum-free media is essential for the development of cell-cultured meat technologies. While some effective media have been developed for relevant cells (e.g., bovine muscle stem cells), these rely on costly recombinant albumin which is too expensive for a scalable system. In this study, we explored bulk protein isolates from rapeseed, cottonseed, and soybean as alternatives to recombinant albumin, and show that rapeseed protein isolate (RPI) is effective in replacing albumin for the serum-free culture of bovine muscle stem cells. We additionally performed proteomic characterization of the three oilseed protein isolates used in the study to compare how proteomic differences might be associated with functional differences between these proteins.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:07.234.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Stout
SpeciesList	scientific name: Gossypium hirsutum; NCBI TaxID: 3635; scientific name: Brassica napus (Rape); NCBI TaxID: 3708; scientific name: Glycine max; NCBI TaxID: 3847;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-05 09:13:23	ID requested
1	2023-03-25 04:49:17	announced
⏵ 2	2023-11-14 08:58:07	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: rapeseed protein isolate, serum-free cell culture, culture media, soybean protein isolate, albumin,Cultured meat, cottonseed protein isolate

David L. Kaplan
contact affiliation	Biomedical Engineering, Tufts University, Medford, MA
contact email	david.kaplan@tufts.edu
lab head
Andrew Stout
contact affiliation	Tufts University
contact email	andrew.stout@tufts.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036518

PRIDE project URI

• PRIDE
  1. PXD036518
     1. Label: PRIDE project
     2. Name: Oilseed protein isolates as albumin alternatives for muscle cell culture media