PXD036514

PXD036514 is an original dataset announced via ProteomeXchange.

Title	Identification of substrates of LarA-activated Lon protease and Identification of Lon protease interactome
Description	With this analysis we aim to identify proteins that are substrates of LarA-activated Lon protease in Caulobacter crescentus. We will compare samples of wild type cells either harboring an empty vector (vector control, VC) or overexpressing 3xFLAG-tagged LarA (F-LarA). Based on our previous data we hypothesize that proteins affected by LarA-overexpression which results in Lon protease activation will decrease in levels compared to the vector control sample. The aim of this analysis is the identification of the interactome of the Lon protease in Caulobacter crescentus. It is a follow-up analysis of a project in which we were looking for novel substrates of the Lon protease in Caulobacter crescentus. To narrow down the list of potential substrates we obtained through the first analysis, we have purified the protease (Lon WT) as well an inactive TRAP mutant and interacting proteins using a Twin-Strep-tag (Iba lifesciences). As a control, a cell lysate without Strep-tag containing protein was included in the purification set-up. The aim is to identify the proteins that specifically interact with the Lon protease by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2023-10-25
AnnouncementXML	Submission_2023-10-25_01:37:54.701.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	GeorgiosMermelekas
SpeciesList	scientific name: Caulobacter crescentus (strain NA1000 / CB15N); NCBI TaxID: 565050;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-05 09:11:17	ID requested
⏵ 1	2023-10-25 01:37:55	announced

Dataset with its publication pending

submitter keyword: Caulobacter crescentus, Lon activity regulator, LarA, Lon protease

JanneLehtiö
contact affiliation	Dept. Oncology Pathology, Karolinska Institutet, and Scilifelab, Stockholm, Sweden
contact email	janne.lehtio@ki.se
lab head
GeorgiosMermelekas
contact affiliation	Karolinska Institutet
contact email	georgios.mermelekas@scilifelab.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD036514

PRIDE project URI

• PRIDE
  1. PXD036514
     1. Label: PRIDE project
     2. Name: Identification of substrates of LarA-activated Lon protease and Identification of Lon protease interactome