PXD036510

PXD036510 is an original dataset announced via ProteomeXchange.

Title	Protein mass Protein mass spectrometry was used to detect the degree of biotinylation of V5-APEX2-HBC-NES after adding H2O2, and to search for host factors that interact with HBV core protein.spectrometry was used to detect the degree of biotinylation of V5-APEX2-HBC-NES after adding H2O2, and to search for host factors that interact with HBV core protein.
Description	Chronic hepatitis B virus (HBV) infection is a leading cause of liver cirrhosis and liver cancer, representing a global health problem for which a functional cure is difficult to achieve. The HBV core protein (HBc) is essential for multiple steps in the viral life cycle; it is the building block of the nucleocapsid in which viral DNA reverse transcription occurs, and it mediates viral–host cell interactions critical to HBV infection persistence. However, systematic studies targeting HBc-interacting proteins remain lacking. An engineered ascorbate peroxidase called APEX2, is genetically targeted to a cellular region of interest and biochemically labeled neighboring proteins within living cells. Cells are then lysed, and biotinylated proteins are enriched with streptavidin beads and identified by mass spectrometry.Here, we combined HBc with the engineered ascorbate peroxidase 2 (APEX2) to systematically identify HBc-related proteins in living cells.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:08:02.549.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liu Yu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-05 09:08:52	ID requested
⏵ 1	2025-05-06 09:08:03	announced

Liu Y, Yang J, Wang Y, Zeng Q, Fan Y, Huang A, Fan H, The proteasome activator subunit PSME1 promotes HBV replication by inhibiting the degradation of HBV core protein. Genes Dis, 11(6):101142(2024) [pubmed]

10.1016/j.gendis.2023.101142;

submitter keyword: LC-MS/MS HBV HBC APEX2

Liu Yu
contact affiliation	The Key Laboratory of Molecular Biology of Infectious Diseases designated by the Chinese Ministry of Education, Chongqing Medical University, China
contact email	445063273@qq.com
lab head
Liu Yu
contact affiliation	PTM Biolabs inc.
contact email	445063273@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD036510

PRIDE project URI

• PRIDE
  1. PXD036510
     1. Label: PRIDE project
     2. Name: Protein mass Protein mass spectrometry was used to detect the degree of biotinylation of V5-APEX2-HBC-NES after adding H2O2, and to search for host factors that interact with HBV core protein.spectrometry was used to detect the degree of biotinylation of V5-APEX2-HBC-NES after adding H2O2, and to search for host factors that interact with HBV core protein.