PXD036508 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The metabolite-controlled ubiquitin conjugase Ubc8 promotes mitochondrial protein import by enhancing assembly of the TOM complex - DATA SET II |
Description | Mitochondria are essential organelles that play a key role in energy metabolism. Transitions between glycolytic and respiring conditions induce considerable adaptations of the cellular proteome. These metabolism-dependent changes are particularly pronounced for the protein composition of mitochondria. Here we show that the ubiquitin conjugase Ubc8 of the yeast cytosol plays a crucial role in the remodeling process when cells are shifted from respiratory to fermentative conditions. Ubc8 is a conserved and well-studied component of the catabolite control system that is known to regulate the stability of gluconeogenesis enzymes. Unexpectedly, we found that Ubc8 also promotes the assembly of the translocase of the outer membrane of mitochondria (TOM) and stabilizes its cytosol-exposed receptor subunit Tom22. Ubc8 deficiency results in a compromised protein import into mitochondria and a subsequent accumulation of mitochondrial precursor proteins in the cytosol. Our observations show that Ubc8 which is controlled by the prevalent metabolic conditions, promotes on the one hand the switch from glucose synthesis to glucose usage in the cytosol and, on the other hand, the biogenesis of the mitochondrial TOM machinery in order to protect the mitochondrial import machinery during phases of metabolic transition. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:57:43.516.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Markus Räschle |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-09-05 09:02:23 | ID requested | |
1 | 2022-10-14 17:33:08 | announced | |
⏵ 2 | 2023-11-14 08:57:43 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mitochondrial import, ubc8, yeast metabolism |
Contact List
Markus Raeschle |
contact affiliation | Molecular Genetics, TU Kaiserdslautern |
contact email | raeschle@bio.uni-kl.de |
lab head | |
Markus Räschle |
contact affiliation | Technical University Kaiserslautern |
contact email | raeschle@rhrk.uni-kl.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD036508
- Label: PRIDE project
- Name: The metabolite-controlled ubiquitin conjugase Ubc8 promotes mitochondrial protein import by enhancing assembly of the TOM complex - DATA SET II