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PXD036504

PXD036504 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe inhibition of putrescine synthesis affects the in vitro shoot development of Cedrela fissilis Vell. (Meliaceae) by altering endogenous polyamine metabolism and the proteomic profile
DescriptionPolyamines (PAs) and proteins have been demonstrated to be fundamental for in vitro shoot development of Cedrela fissilis. We evaluated the influence of 6-benzyladenine (BA) and putrescine (Put) on the growth of shoots, PA metabolism and proteomic profiles of C. fissilis. The longest shoots were obtained under 2.5 μM BA + 2.5 mM Put treatment. The inhibition of Put synthesis by D-arginine (D-arg) decreased the shoot length and reduced the endogenous contents of free Put and the activities of the Put biosynthesis enzymes arginine decarboxylase (ADC) and ornithine decarboxylase (ODC), resulting in reduced shoot growth. The ODC activity was higher than that of ADC, which is the main enzyme in the synthesis of Put in C. fissilis. Inhibition of Put synthesis affected the proteomic profile, reducing the accumulation of the ubiquitin receptor RAD23c, peroxidase 15, ADP-ribosylation factor 1, ADP-ribosylation factor-like protein 8a, profilin-4, profilin-2, glucan endo-1,3-beta-glucosidase, and expansin-like B1 and increasing the accumulation of V-type proton ATPase catalytic subunit A and methionine gamma-lyase, highlighting the relevance of these proteins in increasing shoot length. The transport protein SEC13 homolog B and the basic isoform glucan endo-1,3-beta-glucosidase, which are unique to shoots treated with BA+Put, are related to the promotion of shoot growth. Our results show that the modulation of endogenous PAs and proteomic profiles is necessary to regulate in vitro morphogenesis in C. fissilis. Moreover, the ODC enzyme is highly involved in the synthesis of Put during in vitro shoot development and is described for the first time in this species.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:02:42.451.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterClaudete Santa-Catarina
SpeciesList scientific name: Cedrela fissilis; NCBI TaxID: 281395;
ModificationListNo PTMs are included in the dataset
InstrumentSYNAPT G2-Si
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-05 08:52:30ID requested
12023-09-06 09:58:51announced
22023-11-14 07:56:33announced2023-11-14: Updated project metadata.
32024-10-22 06:02:42announced2024-10-22: Updated project metadata.
Publication List
10.1007/S11240-022-02414-1;
Keyword List
submitter keyword: Polyamine inhibitor, Morphogenic competence, Enzymatic activities,Putrescine, Proteomics
Contact List
Claudete Santa Catarina
contact affiliationUniversidade Estadual do Norte Fluminense Darcy Ribeiro Laboratório de Biologia Celular ou Tecidual Av. Alberto Lamego 2000 - Campos dos Goytacazes-RJ 28013-602 - Brazil (lab head)
contact emailclaudete@uenf.br
lab head
Claudete Santa-Catarina
contact affiliationUniversidade Estadual do Norte Fluminense Darcy Ribeiro
contact emailclaudete@uenf.br
dataset submitter
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