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PXD036482

PXD036482 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHPLC-TIMS-TOF-MS of purified mitochondrial I+III2 supercomplex from Arabidopsis thaliana
DescriptionMitochondrial I+III2 supercomplex was purified from Arabidopsis thaliana cells using differential centrifugation including Percoll step gradients and continuous sucrose gradients. Supercomplex fractions were digested with trypsin using the SP3 approach. Peptides were further purified with SepPak cartridges and measured by HPLC-ESI-TIMS-TOF-MS. Database search was performed with MaxQuant (Version 2.0.3.0.) Over 1,300 proteins were identified using this highly sensitive approach, including all known subunits of the I+III2 supercomplex. This MS analysis is part of a publication on the structure of the I+III2 supercomplex from Arabidopsis by Klusch et al., 2022.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:59:20.985.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNils Rugen
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-02 06:48:14ID requested
12022-12-30 11:59:35announced
22023-11-14 08:59:21announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Arabidopsis, supercomplex, mitochondria
Contact List
Hans-Peter Braun
contact affiliationProf. Dr. Hans-Peter Braun Abteilung Pflanzenproteomik Institut für Pflanzengenetik Naturwissenschaftliche Fakultät Leibniz Universität Hannover Herrenhäuser Str. 2 30419 Hannover Germany
contact emailbraun@genetik.uni-hannover.de
lab head
Nils Rugen
contact affiliationInstitut für Pflanzengenetik Leibniz Universität Hannover Herrenhäuser Str. 2 30419 Hannover Germany
contact emailrugen@genetik.uni-hannover.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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