PXD036482

PXD036482 is an original dataset announced via ProteomeXchange.

Title	HPLC-TIMS-TOF-MS of purified mitochondrial I+III2 supercomplex from Arabidopsis thaliana
Description	Mitochondrial I+III2 supercomplex was purified from Arabidopsis thaliana cells using differential centrifugation including Percoll step gradients and continuous sucrose gradients. Supercomplex fractions were digested with trypsin using the SP3 approach. Peptides were further purified with SepPak cartridges and measured by HPLC-ESI-TIMS-TOF-MS. Database search was performed with MaxQuant (Version 2.0.3.0.) Over 1,300 proteins were identified using this highly sensitive approach, including all known subunits of the I+III2 supercomplex. This MS analysis is part of a publication on the structure of the I+III2 supercomplex from Arabidopsis by Klusch et al., 2022.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:20.985.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nils Rugen
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-02 06:48:14	ID requested
1	2022-12-30 11:59:35	announced
⏵ 2	2023-11-14 08:59:21	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Arabidopsis, supercomplex, mitochondria

Hans-Peter Braun
contact affiliation	Prof. Dr. Hans-Peter Braun Abteilung Pflanzenproteomik Institut für Pflanzengenetik Naturwissenschaftliche Fakultät Leibniz Universität Hannover Herrenhäuser Str. 2 30419 Hannover Germany
contact email	braun@genetik.uni-hannover.de
lab head
Nils Rugen
contact affiliation	Institut für Pflanzengenetik Leibniz Universität Hannover Herrenhäuser Str. 2 30419 Hannover Germany
contact email	rugen@genetik.uni-hannover.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD036482

PRIDE project URI

• PRIDE
  1. PXD036482
     1. Label: PRIDE project
     2. Name: HPLC-TIMS-TOF-MS of purified mitochondrial I+III2 supercomplex from Arabidopsis thaliana