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PXD036476

PXD036476 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of hepatocytes secretomes after alpha-amanitin exposure by quantitative proteomics
DescriptionIn the human body, proteins secreted into peripheral blood vessels are known as the secretome, and they serve as a representation of the physiological or pathological status of cells. The unique response of cells to toxin exposure can be confirmed via secretome analysis, which can be applied to discover toxic mechanisms or exposure markers. The most widely reported amatoxin is alpha-amanitin (-AMA), which inhibits transcription and protein synthesis by directly interacting with RNA polymerase II. However, the secretory proteins released by hepatic failure caused by -AMA are still not fully characterized. In this study, we analyzed secretory proteins in -AMA-induced hepatotoxicity after -AMA treatment to Huh-7 cells using a comparative proteomics technique. In the results, 1,505 and 1,440 were identified and quantified and significantly up- and down-regulated proteins were 4 and 127 proteins, respectively. Then, bioinformatics analysis was performed to get the GO, Interpro and KEGG pathways about significantly down-regulated proteins. Based on bioinformatics results, we picked complement component 3 (C3) as an exposure marker after -AMA-induced hepatotoxicity. Through western blot and C3 ELISA assay, we validated the C3 level was decreased resulting from -AMA. In conclusion, we found the C3 level in secretomes reduced after -AMA-induced hepatotoxicity using comparative proteomics and molecular biology techniques. We expect this research will be helpful to find new toxic mechanisms, therapeutic targets, and exposure markers in -AMA-induced hepatotoxicity.
HostingRepositoryPRIDE
AnnounceDate2024-05-23
AnnouncementXMLSubmission_2024-05-22_23:36:26.250.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDoeun Kim
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-09-02 06:35:01ID requested
12024-05-22 23:36:27announced
Publication List
Kim D, Lee MS, Sim H, Lee S, Lee HS, Characterization of complement C3 as a marker of alpha-amanitin toxicity by comparative secretome profiling. Toxicol Res, 39(2):251-262(2023) [pubmed]
10.1007/s43188-022-00163-z;
Keyword List
submitter keyword: alpha-amanitin
toxic mushroom
secretomes
exposure marker
Complement component 3
Contact List
Sangkyu Lee
contact affiliationBK21 FOUR Community-Based Intelligent Novel Drug Discovery Education Unit, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University
contact emailsangkyu@knu.ac.kr
lab head
Doeun Kim
contact affiliationCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 41566, Republic of Korea
contact emailkdkdl1230@gmail.com
dataset submitter
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