PXD036476

PXD036476 is an original dataset announced via ProteomeXchange.

Title	Characterization of hepatocytes secretomes after alpha-amanitin exposure by quantitative proteomics
Description	In the human body, proteins secreted into peripheral blood vessels are known as the secretome, and they serve as a representation of the physiological or pathological status of cells. The unique response of cells to toxin exposure can be confirmed via secretome analysis, which can be applied to discover toxic mechanisms or exposure markers. The most widely reported amatoxin is alpha-amanitin (-AMA), which inhibits transcription and protein synthesis by directly interacting with RNA polymerase II. However, the secretory proteins released by hepatic failure caused by -AMA are still not fully characterized. In this study, we analyzed secretory proteins in -AMA-induced hepatotoxicity after -AMA treatment to Huh-7 cells using a comparative proteomics technique. In the results, 1,505 and 1,440 were identified and quantified and significantly up- and down-regulated proteins were 4 and 127 proteins, respectively. Then, bioinformatics analysis was performed to get the GO, Interpro and KEGG pathways about significantly down-regulated proteins. Based on bioinformatics results, we picked complement component 3 (C3) as an exposure marker after -AMA-induced hepatotoxicity. Through western blot and C3 ELISA assay, we validated the C3 level was decreased resulting from -AMA. In conclusion, we found the C3 level in secretomes reduced after -AMA-induced hepatotoxicity using comparative proteomics and molecular biology techniques. We expect this research will be helpful to find new toxic mechanisms, therapeutic targets, and exposure markers in -AMA-induced hepatotoxicity.
HostingRepository	PRIDE
AnnounceDate	2024-05-23
AnnouncementXML	Submission_2024-05-22_23:36:26.250.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Doeun Kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-02 06:35:01	ID requested
⏵ 1	2024-05-22 23:36:27	announced

Kim D, Lee MS, Sim H, Lee S, Lee HS, Characterization of complement C3 as a marker of alpha-amanitin toxicity by comparative secretome profiling. Toxicol Res, 39(2):251-262(2023) [pubmed]

10.1007/s43188-022-00163-z;

submitter keyword: alpha-amanitin

toxic mushroom

secretomes

exposure marker

Complement component 3

Sangkyu Lee
contact affiliation	BK21 FOUR Community-Based Intelligent Novel Drug Discovery Education Unit, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University
contact email	sangkyu@knu.ac.kr
lab head
Doeun Kim
contact affiliation	College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 41566, Republic of Korea
contact email	kdkdl1230@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD036476

PRIDE project URI

• PRIDE
  1. PXD036476
     1. Label: PRIDE project
     2. Name: Characterization of hepatocytes secretomes after alpha-amanitin exposure by quantitative proteomics