PXD036457

PXD036457 is an original dataset announced via ProteomeXchange.

Title	Super-killer CTLs are generated by single gene deletion of Bach2
Description	Bach2 codes for a transcriptional regulator exerting major influences on T cell mediated immune regulation. Effector CTLs derived from in vitro activation of murine CD8+ T cells showed increased proliferative and cytolytic capacity in the absence of BACH2. Before activation, BACH2-deficient CD8+ T cells had a higher abundance of memory and reduced abundance of naïve cells compared to wild-type. CTLs derived from central memory T cells were more potently cytotoxic than those derived from naïve T cells, but even within separated subsets, BACH2-deficiency conferred a cytotoxic advantage. Immunofluorescence and electron microscopy revealed larger granules in BACH2-deficient compared to wild-type CTLs, and proteomic analysis showed an increase in granule content, including perforin and granzymes. Thus, the enhanced cytotoxicity observed in effector CTLs lacking BACH2 arises not only from differences in their initial differentiation state but also inherent production of enlarged cytolytic granules. These results demonstrate how a single gene deletion can produce a CTL super-killer.
HostingRepository	PRIDE
AnnounceDate	2022-09-02
AnnouncementXML	Submission_2022-09-02_07:01:41.961.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alejandro Brenes
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-02 05:13:12	ID requested
⏵ 1	2022-09-02 07:01:42	announced

Dataset with its publication pending

submitter keyword: CTL, T cell, cd8, proteomics, tmt, bach2

Doreen A Cantrell
contact affiliation	Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee
contact email	d.a.cantrell@dundee.ac.uk
lab head
Alejandro Brenes
contact affiliation	University of Dundee
contact email	ajbrenesmurillo@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD036457

PRIDE project URI

• PRIDE
  1. PXD036457
     1. Label: PRIDE project
     2. Name: Super-killer CTLs are generated by single gene deletion of Bach2