PXD036449

PXD036449 is an original dataset announced via ProteomeXchange.

Title	Metabolic clogging of mannose in human cancer cells triggers genome instability via dNTP loss
Description	Mannose is an anti-cancer sugar that inhibits cell proliferation and enhances chemotherapy. How mannose exerts its anti-cancer activities, however, remains poorly understood. Here, using genetically engineered human cancer cells that permit the precise control of mannose metabolic flux, we demonstrate that the large influx of mannose exceeding its metabolic capacity induced a global metabolic remodeling, leading to the generation of slow-cycling cells with limited deoxyribonucleoside triphosphate (dNTP) pools. This metabolic remodeling impaired dormant origin firing required to rescue stalled forks by cisplatin, thus exacerbating replication stress. Importantly, a pharmacological inhibition of de novo dNTP biosynthesis was sufficient to sensitize the non-engineered cells to cisplatin and inhibit dormant origin firing, suggesting the dNTP loss-induced genome instability as a major mechanism for the anti-cancer activities of mannose.
HostingRepository	PRIDE
AnnounceDate	2023-07-06
AnnouncementXML	Submission_2023-07-05_17:19:44.934.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036449
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	TakehiroSuzuki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive HF-X; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-02 04:55:47	ID requested
⏵ 1	2023-07-05 17:19:45	announced

10.6019/PXD036449;

submitter keyword: Mannose cancer

NaoshiDohmae
contact affiliation	Biomolecular characterization unit, Center for Sustainable Resource Science, RIKEN
contact email	dohmae@riken.jp
lab head
TakehiroSuzuki
contact affiliation	RIKEN Center for Sustainable Resource Science
contact email	takehirosuzuki@riken.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036449
     1. Label: PRIDE project
     2. Name: Metabolic clogging of mannose in human cancer cells triggers genome instability via dNTP loss