PXD036445

PXD036445 is an original dataset announced via ProteomeXchange.

Title	Consistency across multi-omics layers in a drug-perturbed gut microbial community
Description	Here we map the molecular response of a synthetic community of 32 human gut bacteria to three non-antibiotic drugs by using five omics layers, namely 16S rRNA gene profiling, metagenomics, metatranscriptomics, metaproteomics, and metabolomics. Using this controlled setting, we find that all omics methods with species resolution in their readouts are highly consistent in estimating relative species abundances across conditions. Furthermore, different omics methods can be complementary in their ability to capture functional changes in response to the drug perturbations. For example, while nearly all omics data types captured that the antipsychotic drug chlorpromazine selectively inhibits Bacteroidota representatives in the community, the metatranscriptome and metaproteome suggested that the drug induces stress responses related to protein quality control and metabolomics revealed a decrease in polysaccharide uptake, likely caused by Bacteroidota depletion. Taken together, our study provides insights into how multi-omics datasets can be utilised to reveal complex molecular responses to external perturbations in microbial communities.
HostingRepository	PRIDE
AnnounceDate	2022-12-13
AnnouncementXML	Submission_2022-12-13_07:12:04.172.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PeterTreit
SpeciesList	scientific name: metagenome; NCBI TaxID: 256318;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-09-01 02:10:19	ID requested
⏵ 1	2022-12-13 07:12:04	announced

Dataset with its publication pending

submitter keyword: Multi-omics proteomics metagenomics metatranscriptomics community drug chlorpromazine niclosamide metformin gut microbiome microbiota

MatthiasMann
contact affiliation	Max Planck Institute for Biochemistry
contact email	mmann@biochem.mpg.de
lab head
PeterTreit
contact affiliation	Max Planck Institut
contact email	treit@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036445
     1. Label: PRIDE project
     2. Name: Consistency across multi-omics layers in a drug-perturbed gut microbial community