PXD036437

PXD036437 is an original dataset announced via ProteomeXchange.

Title	Senolytics improve the neurogenic potential of non-glial progenitors and neurorepair in the dorsal pallium of aged killifish independent of inflammation
Description	With advancing age, senescent cells accumulate as they are not efficiently cleared by the immune system anymore. Via a senescence-associated secretory phenotype, chronic senescent cells alter the microenvironment, creating an unfavorable milieu for neurogenesis and neurorepair. Using an innovative and rapid aging model, the African turquoise killifish, we have previously demonstrated a dramatic decline in neurogenic potential of non-glial progenitors with age. Even after traumatic brain injury, progenitor proliferation and neuron production was very low in aged killifish in comparison to young adult killifish, and overall neurorepair was incomplete. In the present study, we validated if the senolytic cocktail dasatinib and quercetin (D+Q) could reboot the neurogenic output by clearing chronic senescent cells from the aged killifish brain to re-create the necessary supportive environment. Our results confirm that the aged killifish telencephalon holds a very high senescent cell burden, which we could diminish by short-term systemic D+Q treatment. As a consequence of D+Q administration, proliferation of non-glial progenitors increased and more new neurons were generated and migrated into the parenchyma after injury. Injury-induced inflammation and glial scarring, a phenomenon only seen in aged killifish, remained unaltered. Senolytic treatment with D+Q might thus hold promise for improving brain function in aged populations, and is especially interesting for reviving the neurogenic potential of an already aged central nervous system.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:25.635.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036437
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kurt Boonen
SpeciesList	scientific name: Nothobranchius furzeri; NCBI TaxID: 105023;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-31 11:28:13	ID requested
1	2023-07-20 10:35:31	announced
⏵ 2	2023-11-14 09:02:33	announced	2023-11-14: Updated project metadata.

Van Houcke J, Mari, ë, n V, Zandecki C, Ayana R, Pepermans E, Boonen K, Seuntjens E, Baggerman G, Arckens L, A short dasatinib and quercetin treatment is sufficient to reinstate potent adult neuroregenesis in the aged killifish. NPJ Regen Med, 8(1):31(2023) [pubmed]

10.6019/PXD036437;

submitter keyword: D+Q, senolytics, neuroregeneration, senescent cells, neurorepair,Aging, N. furzeri

Lut Arckens
contact affiliation	Laboratory of Neuroplasticity and Neuroproteomics, Department of Biology, KU Leuven, 3000 Leuven, Belgium
contact email	lut.arckens@kuleuven.be
lab head
Kurt Boonen
contact affiliation	University of Antwerp, VITO
contact email	kurt.boonen@uantwerpen.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036437
     1. Label: PRIDE project
     2. Name: Senolytics improve the neurogenic potential of non-glial progenitors and neurorepair in the dorsal pallium of aged killifish independent of inflammation