PXD036432

PXD036432 is an original dataset announced via ProteomeXchange.

Title	TISSUE AND URINARY PROTEOMIC ANALYSIS OF KIDNEYS UNDERGOING SHORT PERIOD OF NORMOTHERMIC MACHINE PERFUSION.
Description	To identify the main biological effects of the normothermic machine perfusion (NMP) in marginal kidneys, we measured, by mass spectrometry analysis, changes in the proteomic profile of kidney tissues and urines of eight organs reconditioned for 120 minutes (by a Kidney Assist device). Biopsy specimens were taken at the time of the pre-implantation histological evaluation (T-1), at the start of back table preparation (T0), and after 60 (T60) and 120(T120) minutes. Urine were collected at T0, T30, T60 and T120. Several bioinformatics/statistical algorithms (including SVM and PLS-DA) were used to select the most discriminative proteins. Bioinformatics analysis showed that, during NMP, a large number of proteins resulted down- or up-regulated in the kidneys (169 and 196, respectively). SVM and PLS-DA analysis restricted this panel to 50 top-discriminative proteins. Among these, 11 proteins resulted similarly up-regulated(LXN, ETFB, NUDT3, CYCS and UQCRC1)and down-regulated after NMP treatment (CFHR3, C1S, CFI, KNG1, SERPINC1, F9)in urine.Functional analysis, then, revealed that the most up-regulated proteins were involved in the oxidative phosphorylation system (OXPHOS) and ATPsynthesis, while thoseunder-expressed were implicated in the complement and coagulation cascade.Our proteomic analysis demonstrated that NMP (also for a brief period of time) may induce substantial metabolic and biochemical changes in marginal organs. This encourages use of this technology in clinic.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:45:56.690.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Bartolucci
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-31 11:20:31	ID requested
1	2023-05-10 09:39:05	announced
⏵ 2	2023-11-14 08:46:01	announced	2023-11-14: Updated project metadata.

Zaza G, Neri F, Bruschi M, Granata S, Petretto A, Bartolucci M, di Bella C, Candiano G, Stallone G, Gesualdo L, Furian L, Proteomics reveals specific biological changes induced by the normothermic machine perfusion of donor kidneys with a significant up-regulation of Latexin. Sci Rep, 13(1):5920(2023) [pubmed]

submitter keyword: normothermic machine perfusion, proteomics, mass spectrometry, urines, kidney

Andrea Petretto
contact affiliation	IRCCS Istituto G Gaslini
contact email	a.petretto@gmail.com
lab head
Martina Bartolucci
contact affiliation	IRCCS Gaslini
contact email	smartibartolucci@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036432
     1. Label: PRIDE project
     2. Name: TISSUE AND URINARY PROTEOMIC ANALYSIS OF KIDNEYS UNDERGOING SHORT PERIOD OF NORMOTHERMIC MACHINE PERFUSION.