PXD036413

PXD036413 is an original dataset announced via ProteomeXchange.

Title	Dysregulation of Wnt/β-Catenin Signaling pathway via RNF146 upregulation in the prefrontal cortex is responsible for social deficit in a VPA-induced ASD mouse model
Description	Autism spectrum disorder (ASD) is a heterogeneous neurodevelopmental disorder where patients have impaired social behavior, communication, repetitive behaviors, and restricted interest. Valproic acid (VPA) has been widely used to treat patients with epilepsy and bipolar disorder patients. However, VPA treatment during pregnancy has produced offspring with neurodevelopmental disorders, including ASD. To better understand the molecular function of ASD, we have used valproic acid, chemically induced ASD mice. We have performed LC-MS/MS analysis of VPA-induced offspring mice to the control to see the difference in their proteome difference. Our analysis showed that many neuronal network pathways were highly expressed in our differentially expressed proteins, and among them, Wnt/ β-catenin pathways showed clear enrichment. The RNF146 (E3 Ubiquitin-protein ligase) increase in VPA-exposed mice showed a highly significant effect on canonical Wnt/ β-catenin signaling pathways by disrupting the β-catenin destruction complex. The proteins like CREBBP, TCF4, and GSK3B also showed significant changes that indicate dysfunction of β-catenin destruction complex and activation of transcription factors.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:40:26.807.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036413
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Wooyoung Jang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-30 14:27:32	ID requested
1	2023-07-24 01:18:51	announced
⏵ 2	2023-11-14 06:40:27	announced	2023-11-14: Updated project metadata.

10.6019/PXD036413;

submitter keyword: mouse model, Valproic acid, VPA, Wnt signaling, Rnf146,Autism spectrum disorder, beta-catenin

Min-Sik Kim
contact affiliation	Professor at Daegu Gyeongbuk Science & Technology (DGIST), New Biology Department.
contact email	mkim@dgist.ac.kr
lab head
Wooyoung Jang
contact affiliation	kyung hee university
contact email	jangwooyoung92@khu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036413
     1. Label: PRIDE project
     2. Name: Dysregulation of Wnt/β-Catenin Signaling pathway via RNF146 upregulation in the prefrontal cortex is responsible for social deficit in a VPA-induced ASD mouse model