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PXD036410

PXD036410 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of proteins interacting with the NEK1-C21ORF2 complex
DescriptionWe and others have found that the NEK1 kinase interacts with the C21ORF2 protein. Gel filtration indicated there may be other proteins in the complex. We tried to identify such proteins by immunoprecipitating endogenous NEK1 from cells and subjecting the precipitates to mass spectrometry. We also immunoprecipitated endogenous NEK1 from cells and subjected the precipitates to mass spectrometry. To find the full complement of NEK1 interactors, NEK1 was immunoprecipitated from ARPE-19 parental cells, and NEK1-KO cells were to control for non-specific binding. Five biological replicates were used per cell line, and after trypsinization of the precipitates, peptides were labelled with tandem mass tags (TMT) enabling quantitative mass spectrometric analysis of the 10 samples which were pooled and analysed in parallel. To find the full complement of C21ORF2 interactors, C21ORF2 was immunoprecipitated from ARPE-19 parental cells, and C21ORF2-KO cells were to control for non-specific binding. Five biological replicates were used per cell line, and after trypsinization of the precipitates, peptides were labelled with tandem mass tags (TMT) enabling quantitative mass spectrometric analysis of the 10 samples which were pooled and analysed in parallel.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:58:05.839.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrederic Lamoliatte
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-30 14:26:11ID requested
12023-05-09 01:18:21announced
22023-11-14 08:58:06announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: C21ORF2, DNA repair, ciliogenesis,NEK1, kinase
Contact List
John Rouse
contact affiliationMRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dundee, UK
contact emailj.rouse@dundee.ac.uk
lab head
Frederic Lamoliatte
contact affiliationUniversity of Dundee
contact emailflamoliatte001@dundee.ac.uk
dataset submitter
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Dataset FTP location
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