PXD036402

PXD036402 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of swine macrophages after infection with two genotype II African swine fever isolates of different pathogenicity
Description	ASFV poses a continuing threat to the pig industry in Europe as broadly applicable vaccines are not available. Variations within the ASFV genome resulted in the emergence of attenuated strains with low or moderate virulence, however, the molecular basis of the differences in virulence is not known. To reveal virulence-associated protein expression patterns, we have analyzed the proteomes of the natural target cells of ASFV, primary porcine macrophages, after infection with two genotype II ASFV strains displaying high (“Armenia 2008”) and moderate (”Estonia 2014”) virulence using quantitative mass spectrometry. Very similar expression patterns were observed for the viral genes. In addition to the canonical ASFV proteins, twelve novel protein products from recently described transcripts could be confirmed in both isolates. Pathway analysis showed that both isolates evoked a similar host proteome response despite their difference in virulence. However, subtle differences in the manipulation of the proteins involved in the proinflammatory response mediated by the MAPK14/p38 signalling cascade were observed.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:34:12.317.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036402
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Axel Karger
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-30 08:24:59	ID requested
1	2023-01-05 06:12:51	announced
⏵ 2	2023-11-14 08:34:13	announced	2023-11-14: Updated project metadata.

10.6019/PXD036402;

W, ö, hnke E, Cackett G, Werner F, Blome S, Mettenleiter TC, Karger A, Proteome Analysis of Swine Macrophages after Infection with Two Genotype II African Swine Fever Isolates of Different Pathogenicity. Viruses, 14(10):(2022) [pubmed]

submitter keyword: ASFV, pathogenicity, porcine macrophages,African swine fever virus

Axel Karger
contact affiliation	Institute of molecular virology and cell biology, Laboratory for biochemistry and protein analysis, Friedrich Loeffler Institut, Germany
contact email	axel.karger@fli.de
lab head
Axel Karger
contact affiliation	Friedrich-Loeffler-Institut
contact email	axel.karger@fli.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD036402

PRIDE project URI

• PRIDE
  1. PXD036402
     1. Label: PRIDE project
     2. Name: Proteome analysis of swine macrophages after infection with two genotype II African swine fever isolates of different pathogenicity