PXD036395

PXD036395 is an original dataset announced via ProteomeXchange.

Title	Early Signaling Events in Renal Compensatory Hypertrophy Revealed by Multi-Omics
Description	Loss of a kidney results in compensatory growth of the remaining kidney, a phenomenon of considerable clinical importance. However, the mechanisms involved are largely unknown. Here, we used a multi-omic approach in a mouse unilateral nephrectomy model to identify signaling processes associated with compensatory hypertrophy of the renal proximal tubule. Morphometry applied to microdissected proximal tubules showed that growth of the proximal tubule involves a marked, rapid increase in cell volume rather than cell number. Measurements of DNA accessibility (ATAC-seq), transcriptome (RNA-seq) and proteome (quantitative protein mass spectrometry) independently identified patterns of change that are indicative of activation of the lipid-regulated transcription factor, PPARα. Activation of PPARα by fenofibrate administration increased proximal tubule cell size, while genetic deletion of PPARα in mice decreased it. The results indicate that PPARα is an important determinant of proximal tubule cell size and is a likely mediator of compensatory proximal tubule hypertrophy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:46:35.536.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CHIN-RANG YANG
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-30 04:33:44	ID requested
1	2023-06-05 10:25:58	announced
2	2023-11-14 09:00:55	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:46:35	announced	2024-10-22: Updated project metadata.

10.1038/S41467-023-38958-9;

submitter keyword: transcriptome, PPARα, proximal tubule, unilateral nephrectomy, proteome,Hypertrophy

Mark A. Knepper
contact affiliation	Epithelial Systems Biology Laboratory Systems Biology Center Division of Intramural Research National Heart, Lung and Blood Institute Building 10, Room 6N307 Bethesda, Maryland 20895-1603
contact email	knepperm@nhlbi.nih.gov
lab head
CHIN-RANG YANG
contact affiliation	National Institutes of Health, USA
contact email	chin-rang.yang@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD036395

PRIDE project URI

• PRIDE
  1. PXD036395
     1. Label: PRIDE project
     2. Name: Early Signaling Events in Renal Compensatory Hypertrophy Revealed by Multi-Omics