PXD036377
PXD036377 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of extracellular membrane vesicles from Pseudomonas aeruginosa (PaOMVs) |
Description | PaOMVs in the culture supernatant of P. aeruginosa were harvested by ultracentrifugation at 100,000 × g, 4℃ for 90 min using a Himac CP80WX Preparative Ultracentrifuge (HITACHI, Tokyo, Japan). After removal of the supernatant, the pellet was washed twice and suspended in ice-cold sterile phosphate-buffered saline (PBS). PaOMVs were then isolated from other damaged membranes, aggregated proteins and non-membranous proteins by step-gradient ultracentrifugation using OptiPrep™ Density Medium (Sigma Aldrich, St. Louis, MO). After centrifugation at 100,000 × g, 4℃ for 16 h, the substances in all six fractions (F1-F6) were collected, diluted in PBS, and harvested by ultracentrifugation at 100,000 × g, 4℃ for 3 h. The pellet from each fraction was washed twice and resuspended in a suitable volume of ice-cold sterile PBS. |
HostingRepository | jPOST |
AnnounceDate | 2023-07-18 |
AnnouncementXML | Submission_2023-07-17_18:04:47.426.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yota Tatara |
SpeciesList | scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-08-28 20:08:53 | ID requested | |
⏵ 1 | 2023-07-17 18:04:48 | announced |
Publication List
Ishiai T, Subsomwong P, Narita K, Kawai N, Teng W, Suzuki S, Sukchawalit R, Nakane A, Asano K, . Curr Res Microb Sci, 4():100190(2023) [pubmed] |
Keyword List
submitter keyword: extracellular vesicles, Pseudomonas aeruginosa |
Contact List
Krisana Asano | |
---|---|
lab head | |
Yota Tatara | |
contact affiliation | Hirosaki University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001829/ |