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PXD036377

PXD036377 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of extracellular membrane vesicles from Pseudomonas aeruginosa (PaOMVs)
DescriptionPaOMVs in the culture supernatant of P. aeruginosa were harvested by ultracentrifugation at 100,000 × g, 4℃ for 90 min using a Himac CP80WX Preparative Ultracentrifuge (HITACHI, Tokyo, Japan). After removal of the supernatant, the pellet was washed twice and suspended in ice-cold sterile phosphate-buffered saline (PBS). PaOMVs were then isolated from other damaged membranes, aggregated proteins and non-membranous proteins by step-gradient ultracentrifugation using OptiPrep™ Density Medium (Sigma Aldrich, St. Louis, MO). After centrifugation at 100,000 × g, 4℃ for 16 h, the substances in all six fractions (F1-F6) were collected, diluted in PBS, and harvested by ultracentrifugation at 100,000 × g, 4℃ for 3 h. The pellet from each fraction was washed twice and resuspended in a suitable volume of ice-cold sterile PBS.
HostingRepositoryjPOST
AnnounceDate2023-07-18
AnnouncementXMLSubmission_2023-07-17_18:04:47.426.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYota Tatara
SpeciesList scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-28 20:08:53ID requested
12023-07-17 18:04:48announced
Publication List
Ishiai T, Subsomwong P, Narita K, Kawai N, Teng W, Suzuki S, Sukchawalit R, Nakane A, Asano K, . Curr Res Microb Sci, 4():100190(2023) [pubmed]
Keyword List
submitter keyword: extracellular vesicles, Pseudomonas aeruginosa
Contact List
Krisana Asano
lab head
Yota Tatara
contact affiliationHirosaki University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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