PXD036376

PXD036376 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Temporal tracking of the insulin action across the cell surface proteome at a resolution of ten seconds
Description	The ligand-receptor signal occurs on cell surface and rapidly triggers a series of events via surface protein alteration. Here, we for the first time report a proteomic platform based on rapid enzymatic surface labeling (PECSL) method and MS-based label-free quantification, to capture the transiently altered proteins in situ and a perturbation-free manner even at a temporal resolution of ten seconds. With this platform, about one thousand GO-annotated cell surface proteins were reproducibly identified in response to insulin stimuli. Notably, we identified thirty-two insulin-regulated proteins on timescales of ten seconds to two minutes, nearly half of which were annotated as insulin/growth factor interactors. In addition to known insulin-regulated proteins of INSR, IGF1R, TFRC, LNPEP, SORT1, novel insulin-regulated proteins from diverse physiological cellular events, like CNNM3, CPD, MRC2, ECE1, were uncovered and further validated. We believe this platform could be a powerful tool to decode the mechanism of many biological processes involving changes of cell surface proteins.
HostingRepository	jPOST
AnnounceDate	2024-03-26
AnnouncementXML	Submission_2024-03-25_08:00:15.051.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanan Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-28 17:47:13	ID requested
⏵ 1	2024-03-25 08:00:15	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Cell surface, plasma membrane, proteomics, enrichment, insulin

submitter keyword: Cell surface, plasma membrane, proteomics, enrichment, insulin

Contact List

Yanan Li
lab head
Yanan Li
contact affiliation	Dalian Institute of Chemical Physics
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001827/