PXD036368

PXD036368 is an original dataset announced via ProteomeXchange.

Title	Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and nonpregnant mare
Description	Leucyl and cystinyl aminopeptidase (LNPEP/oxytocinase) is a key regulator of oxytocin. Our objectives were to measure serum concentrations of LNPEP and determine the presence of LNPEP and oxytocin (OXT) in equine tissue. LNPEP was measured by ELISA in serum obtained: every other day from ovulation (D0) until D21-22 in nonpregnant and pregnant mares; and at around 320 days of gestation, at 24 hours before foaling, and 20 min and 2 hrs post-foaling. Placenta from the pregnant horn (PH), nonpregnant horn (NPH) and body (B) and from tissues of 6 nonpregnant mares were homogenized and extracts obtained for protein determination and LNPEP ELISA assay. Identification of LNPEP and OXT protein in tissues was also performed via western blot (WB), immunohistochemistry (IHC) and liquid chromatography-mass spectrometry (LC-MS/MS). Furthermore, in situ hybridization was performed for LNPEP and OXT on endometrium, myometrium, pituitary and corpus luteum (CL). No difference in serum LNPEP concentration was identified between any of the groups. The NPH had the lowest quantities of LNPEP (P<0.05). The highest to lowest LNPEP U/mg protein by tissue were: myometrium> follicle wall> endometrium> kidney>CL> liver. Oxytocin was identified in the equine pituitary, CL and placenta. LNPEP may regulate the availability of luteal OXT locally.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:20.722.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariana Diel de Amorim
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-28 03:26:01	ID requested
1	2023-05-10 13:18:56	announced
⏵ 2	2023-11-14 08:46:21	announced	2023-11-14: Updated project metadata.

Diel de Amorim M, Dong L, Byron M, Foster RA, Klein C, Saleh M, Saleh T, Card C, Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and non-pregnant mare. Sci Rep, 13(1):4616(2023) [pubmed]

submitter keyword: oxytocin, reproductive tissue., equine, LNPEP,Corpus luteum, placenta

Mariana Diel de Amorim
contact affiliation	Department of Clinical Sciences, Cornell University, 930 Campus Rd, Ithaca, NY, 14853, USA
contact email	md649@cornell.edu
lab head
Mariana Diel de Amorim
contact affiliation	Department of Clinical Sciences, College of Veterinary Medicine, Cornell University
contact email	md649@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD036368

PRIDE project URI

• PRIDE
  1. PXD036368
     1. Label: PRIDE project
     2. Name: Characterization of serum and tissue oxytocinase and tissue oxytocin in the pregnant and nonpregnant mare