PXD036362

PXD036362 is an original dataset announced via ProteomeXchange.

Title	LC-MS-based detection of silkworm pupae in feed with and without prior immunoaffinity enrichment
Description	Due to their advantageous nutrient composition and high feed conversion efficiency, insects may be an alternative protein source for human and animal nutrition. Recently authorised for feeding purposes in the European Union, silkworm pupae are vastly and cheaply available by-products from sericulture that have been successfully replacing fish meal in aquaculture feed. In order to enable silkworm detection by official control, we firstly identified potential, easily detectable peptide targets from dried silkworm pupae subjected to a standard mass spectrometry (MS)-based bottom-up proteomic workflow. Secondly, a targeted method with an optional immunoaffinity enrichment (IAE) step was developed. Method validation provided a highly reproducible limit of detection of 0.05% (w/w) silkworm pupae in various feeds (aquaculture, poultry, pig) that proved independent from the experimenter and whether IAE was included in sample preparation or not. Furthermore, the method’s specificity and robustness were assessed.
HostingRepository	PRIDE
AnnounceDate	2025-06-05
AnnouncementXML	Submission_2025-06-05_03:55:59.812.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036362
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Uta M. Herfurth
SpeciesList	scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-28 03:23:46	ID requested
⏵ 1	2025-06-05 03:56:00	announced

10.3920/JIFF2022.0098;

10.6019/PXD036362;

submitter keyword: insect,proteomics, feed, Orbitrap, silkworm (Bombyx mori) pupae, LC-MS/MS

Uta Maria
contact affiliation	German Federal Institute for Risk Assessment Department Food Safety National Reference Laboratory for Animal Protein in Feed
contact email	uta.herfurth@bfr.bund.de
lab head
Uta M. Herfurth
contact affiliation	German Federal Institute for Risk Assessment National Reference Laboratory for Animal Proteins in Feed Department Food Safety Max-Dohrn-Straße 8-10, 10589 Berlin, Germany
contact email	uta.herfurth@bfr.bund.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD036362

PRIDE project URI

• PRIDE
  1. PXD036362
     1. Label: PRIDE project
     2. Name: LC-MS-based detection of silkworm pupae in feed with and without prior immunoaffinity enrichment