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PXD036350

PXD036350 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDesign and characterization of mutated variants of the oncotoxic parvoviral protein NS1
DescriptionOncotoxic proteins such as the non-structural protein 1 (NS1), a constituent of the rodent parvovirus H1 (H1-PV), offer a novel approach for treatment of tumors that are refractory to other treatments. In the present study, mutated NS1 variants were designed and tested with respect to their oncotoxic potential in human hepatocellular carcinoma cell lines. We introduced single point mutations of previously described important residues of the wild-type NS1 protein and a deletion of 114 base pairs localized within the N-terminal domain of NS1. Cell-viability screening with HepG2 and Hep3B hepatocarcinoma cells transfected with the constructed NS1-mutants led to identification of, the single-amino acid NS1-mutant NS1-T585E, which lead to a 30% decreased in cell viability as compared to NS1 wildtype. Using proteomics analysis, we could identify new interaction partners and signaling pathways of NS1. We could thus identify new oncotoxic NS1 variants and gain insight into the modes of action of NS1, which is exclusively toxic to human cancer cells. Our in-vitro studies provide mechanistic explanations for the observed oncolytic effects. The availability of new NS1 variants in combination with a better understanding of their modes of action offers new possibilities for the design of innovative cancer treatment strategies.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:12:35.054.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKatarzyna Buczak
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListbiotinylated residue; phosphorylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Orbitrap Fusion Lumos; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-27 06:01:42ID requested
12023-03-10 16:28:22announced
22023-11-14 08:12:37announced2023-11-14: Updated project metadata.
Publication List
Hauswirth P, Graber P, Buczak K, Mancuso RV, Schenk SH, N, ΓΌ, esch JPF, Huwyler J, Design and Characterization of Mutated Variants of the Oncotoxic Parvoviral Protein NS1. Viruses, 15(1):(2023) [pubmed]
Keyword List
submitter keyword: NP1, TMT, LFQ,HCC, parvovirus H-1
Contact List
Alexander Schmidt
contact affiliationProteomics Core Facility, Biozentrum, University of Basel, Switzerland
contact emailalex.schmidt@unibas.ch
lab head
Katarzyna Buczak
contact affiliationBiozentrum, University of Basel
contact emailkatarzyna.buczak@unibas.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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