PXD036344 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry analysis of METTL3 phosphorylation in extracellular growth factor signaling |
Description | A fundamental facet of cell signaling is the conversion of extracellular signals into adaptive transcriptional responses. The role of RNA modifications in this process is poorly understood. We found that epidermal growth factor (EGF) promotes ERK-dependent phosphorylation of the m6A writer enzyme METTL3 at serine-43. Serine-43 phosphorylation increased METTL3-mediated methylation of the small nuclear RNA 7SK. Here we performed of phosphorylation mass spectrometry based on Data independent acquisition (DIA) of Hela cells stimulated with EGF in the absence or presence of the inhibitor U0126. Additionally, we validated known interactions between the 7SK-associated protein LARP7. Our findings establish a novel function for the m6A modification in converting growth-factor signaling events to a transcriptional elongation regulatory response via an RNA-methylation-dependent switch mediated by ERK signaling. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:51:02.975.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wenxue Li |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-26 08:01:48 | ID requested | |
1 | 2023-06-27 12:00:49 | announced | |
⏵ 2 | 2024-10-22 05:51:03 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: HeLa cells, phosphorylation,DIA-MS, METTL3 |
Contact List
Yansheng Liu |
contact affiliation | Department of Pharmacology, Yale University School of Medicine |
contact email | yansheng.liu@yale.edu |
lab head | |
Wenxue Li |
contact affiliation | Yale University |
contact email | wenxue.li@yale.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036344
- Label: PRIDE project
- Name: Mass spectrometry analysis of METTL3 phosphorylation in extracellular growth factor signaling