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PXD036344

PXD036344 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometry analysis of METTL3 phosphorylation in extracellular growth factor signaling
DescriptionA fundamental facet of cell signaling is the conversion of extracellular signals into adaptive transcriptional responses. The role of RNA modifications in this process is poorly understood. We found that epidermal growth factor (EGF) promotes ERK-dependent phosphorylation of the m6A writer enzyme METTL3 at serine-43. Serine-43 phosphorylation increased METTL3-mediated methylation of the small nuclear RNA 7SK. Here we performed of phosphorylation mass spectrometry based on Data independent acquisition (DIA) of Hela cells stimulated with EGF in the absence or presence of the inhibitor U0126. Additionally, we validated known interactions between the 7SK-associated protein LARP7. Our findings establish a novel function for the m6A modification in converting growth-factor signaling events to a transcriptional elongation regulatory response via an RNA-methylation-dependent switch mediated by ERK signaling.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:51:02.975.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWenxue Li
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-26 08:01:48ID requested
12023-06-27 12:00:49announced
22024-10-22 05:51:03announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: HeLa cells, phosphorylation,DIA-MS, METTL3
Contact List
Yansheng Liu
contact affiliationDepartment of Pharmacology, Yale University School of Medicine
contact emailyansheng.liu@yale.edu
lab head
Wenxue Li
contact affiliationYale University
contact emailwenxue.li@yale.edu
dataset submitter
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Dataset FTP location
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