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PXD036339

PXD036339 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHeLa, HEK293T and mouse lung, heart and spleen LC-MS/MS
DescriptionProteins are ubiquitously modified with glycans of varied chemical structures via distinct glycosidic linkages, making the landscape of protein glycosylation challenging to map. Profiling of intact glycopeptides with mass spectrometry (MS) has recently emerged as a powerful tool for revealing matched information of the glycosylation sites and attached glycans (i.e., intact glycosites), which, however, is largely limited to individual glycosylation types. Here, we develop Click-iG, which integrates metabolic labeling of glycans with clickable unnatural sugars, an optimized MS method, and a tailored version of pGlyco3 software to enable simultaneous enrichment and profiling of three types of—N-linked, O-linked, and O-GlcNAcylated—intact glycopeptides. We demonstrated Click-iG by identification of thousands of intact glycosites in cell lines and living mice. From the mouse lung, heart, and spleen, a total of 547 N-glycosylation sites with 161 glycan compositions, 198 O-glycosylation sites with 262 glycans, and 1,947 O‑GlcNAcylation sites were identified. The Click-iG-enabled unprecedent coverage on the protein glycosylation landscape lays the foundation for interrogating crosstalk between different glycosylation pathways.
HostingRepositoryPRIDE
AnnounceDate2025-02-12
AnnouncementXMLSubmission_2025-02-12_08:34:25.795.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJiankun Wang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-08-26 08:00:04ID requested
12025-02-12 08:34:26announced
Publication List
10.1002/anie.202303410;
Liu J, Cheng B, Fan X, Zhou X, Wang J, Zhou W, Li H, Zeng W, Yang P, Chen X, Click-iG: Simultaneous Enrichment and Profiling of Intact N-linked, O-GalNAc, and O-GlcNAcylated Glycopeptides. Angew Chem Int Ed Engl, 62(36):e202303410(2023) [pubmed]
Keyword List
submitter keyword: HeLa, mouse lung, heart, HEK293T, spleen, LC-MS/MS
Contact List
Xing Chen
contact affiliationCollege of Chemistry and Molecular Engineering, Peking University, Beijing, China.
contact emailxingchen@pku.edu.cn
lab head
Jiankun Wang
contact affiliation13020028503
contact email1901111544@pku.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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