PXD036308

PXD036308 is an original dataset announced via ProteomeXchange.

Title	LiP-small molecule mapping of Farrerol
Description	Limited proteolysis combined with mass spectrometry of farrerol treated. Briefly, cell lysates containing approximately 500 μg of total protein sample were incubated with farrerol or vehicle (DMSO) for 10 min at 25 °C. Proteinase K (5 μg, Sigma–Aldrich) was added simultaneously to each sample for the following 10 min. Then, the reaction was stopped by heating at 98 °C for 3 min. The samples were subjected to limited proteolysis to generate structure-specific protein fragments. Complete digestion was performed with trypsin (Promega) at a 1:50 ratio (trypsin:protein, w:w) for 16 h at 37 °C, and then the reaction was stopped by adding formic acid to reach a pH less than 2. The polypeptide samples were dissolved in 0.1% formic acid and detected by Orbitrap mass spectrometry with label-free quantitation (LFQ) analysis to search for differential proteins on the MaxQuant platform via MS sensitivity.
HostingRepository	iProX
AnnounceDate	2022-08-25
AnnouncementXML	Submission_2023-09-11_20:55:39.043.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiwei Song
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-25 19:17:38	ID requested
⏵ 1	2023-09-11 20:55:40	announced

Zhang W, Wang M, Song Z, Fu Q, Chen J, Zhang W, Gao S, Sun X, Yang G, Zhang Q, Yang J, Tang H, Wang H, Kou X, Wang H, Mao Z, Xu X, Gao S, Jiang Y, Farrerol directly activates the deubiqutinase UCHL3 to promote DNA repair and reprogramming when mediated by somatic cell nuclear transfer. Nat Commun, 14(1):1838(2023) [pubmed]

submitter keyword: farrerol, limited protease

Zhiyong Mao
contact affiliation	Tongji University
contact email	zhiyong_mao@tongji.edu.cn
lab head
Zhiwei Song
contact affiliation	Tongji University
contact email	songzhiwei@tongji.edu.cn
dataset submitter

iProX dataset URI