PXD036297

PXD036297 is an original dataset announced via ProteomeXchange.

Title	Generation and Characterization of a Conditional Ku70 Knockout Cell Line
Description	In this study we characterize and investigate the effect of the loss of Ku70 protein in a conditional Ku70 knockout TREx-293 cell system. Loss of Ku70 protein levels was directly correlated with a loss of cell viability, supporting the observation that Ku performs an essential role in human cells. Interestingly, critical loss of average telomere length was not observed in Ku70 knockouts generated. Global quantitative proteomic analysis of whole cell extracts from Ku70 knockouts following depletion of Ku70 may indicate that Ku’s essential role in humans is not at telomeres, but a non-canonical role which is not yet fully understood.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:46:30.980.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Maitland
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-25 14:54:37	ID requested
1	2023-07-27 09:12:16	announced
⏵ 2	2023-11-14 06:46:31	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Ku70 HEK293

Caroline Schild-Poulter
contact affiliation	Department of Biochemistry, Robarts Research Institute, Schulich School of Medicine & Dentistry, Western University
contact email	cschild-poulter@robarts.ca
lab head
Matthew Maitland
contact affiliation	University of Western Ontario
contact email	mmaitla2@uwo.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD036297

PRIDE project URI

• PRIDE
  1. PXD036297
     1. Label: PRIDE project
     2. Name: Generation and Characterization of a Conditional Ku70 Knockout Cell Line