PXD036296

PXD036296 is an original dataset announced via ProteomeXchange.

Title	Analysis of changes in protein expression in inferior colliculus
Description	One of the most robust behavioural phenotypes in the Fmr1-/ymouse is an increased susceptibility to AGS, which models the sensory hypersensitivity and epileptiform activity seen in FX patients (Berry-Kravis, 2002; Musumeci et al., 1991; Yan et al., 2004; Yan et al., 2005). Multiple therapeutic strategies that alleviate AGS have gone on to be tested in clinical studies, suggesting this assay has predictive value (Liu and Smith, 2014; Michalon et al., 2012; Osterweil et al., 2013; Pacey et al., 2009). Recent studies implicate the inferior colliculus (IC) as a central mediator of the AGS phenotype. The IC is a central hub for auditory processing, relaying information from brainstem nuclei to the auditory cortex and other brain regions (Ayala et al., 2016). In the Fmr1-/ymouse, auditory stimulation causes an enhanced activation of IC neurons as assessed by cFos+ staining and in vivosingle-unit recordings (Nguyen et al., 2020). Moreover, studies using conditional expression of Fmr1in specific neuronal populations show that loss of FMRP in glutamatergic VGlut2+ neurons in the IC is both necessary and sufficient for AGS induction (Gonzalez et al., 2019). We aim to test whether changes in proteasome activity is seen in the Fmr1-/y IC, and if so whether modulating UPS activity could resolve pathological changes.
HostingRepository	PRIDE
AnnounceDate	2025-05-02
AnnouncementXML	Submission_2025-05-02_02:30:18.961.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jimi Wills
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-25 14:54:14	ID requested
⏵ 1	2025-05-02 02:30:19	announced

10.1016/j.neuron.2022.11.012;

Louros SR, Seo SS, Maio B, Martinez-Gonzalez C, Gonzalez-Lozano MA, Muscas M, Verity NC, Wills JC, Li KW, Nolan MF, Osterweil EK, Excessive proteostasis contributes to pathology in fragile X syndrome. Neuron, 111(4):508-525.e7(2023) [pubmed]

submitter keyword: AGS,inferior colliculus, proteasome, Fragile X

Emily Osterweil
contact affiliation	Centre for Discovery Brain Sciences, University of Edinburgh, UK; Simons Initiative for the Developing Brain, University of Edinburgh, UK
contact email	Emily.Osterweil@ed.ac.uk
lab head
Jimi Wills
contact affiliation	Firefinch Software Ltd formerly Univeristy of Edinburgh
contact email	jimi@firefinch.io
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD036296

PRIDE project URI

• PRIDE
  1. PXD036296
     1. Label: PRIDE project
     2. Name: Analysis of changes in protein expression in inferior colliculus