PXD036289

PXD036289 is an original dataset announced via ProteomeXchange.

Title	Revealing the effect of SARS-CoV-2 spike stabilisation on receptor binding dynamics with single molecule mass photometry
Description	Here we show using mass photometry how proline substitutions, commonly used for SARS-CoV-2 spike stabilisation and vaccine design, directly impedes ACE2 receptor interactions via dynamics of open and closed states. Conformational changes and ACE2 binding were influenced by spike variant and temperature, but independent of site-specific N-glycosylation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:56:27.765.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sean Burnap
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-25 14:27:46	ID requested
1	2023-06-28 03:08:38	announced
⏵ 2	2023-11-14 06:56:28	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: SARS-CoV-2, Glycomics, Spike, Glycoproteomics, Mass photometry, ACE2

Weston Struwe
contact affiliation	Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, South Parks Road, OX1 3TA.
contact email	weston.struwe@chem.ox.ac.uk
lab head
Sean Burnap
contact affiliation	Kavli Institute for Nanoscience Discovery, University of Oxford, Oxford, UK
contact email	sean.burnap@chem.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD036289

PRIDE project URI

• PRIDE
  1. PXD036289
     1. Label: PRIDE project
     2. Name: Revealing the effect of SARS-CoV-2 spike stabilisation on receptor binding dynamics with single molecule mass photometry