PXD036269

PXD036269 is an original dataset announced via ProteomeXchange.

Title	Generation of a high yield vaccine backbone for influenza B virus in embryonated chicken eggs
Description	Influenza B virus (IBV) strains are one of the components of seasonal influenza vaccines in both trivalent and quadrivalent formulations. The vast majority of these vaccines are produced in embryonated chickens' eggs. While optimized backbones for vaccine production in eggs exist and are in use for influenza A viruses, no such backbones exist for IBVs, resulting in unpredictable production yields. To generate an optimal vaccine seed virus backbone, we have compiled a panel of 71 IBV strains from 1940 to present day, representing the known temporal and genetic variability of IBV circulating in humans. This panel contains strains from the B/Victoria/2/87-like lineage, B/Yamagata/16/88-like lineage and the ancestral lineage that preceded their split to provide a diverse set that would help to identify a suitable backbone which can be used in combination with hemagglutinin (HA) and neuraminidase (NA) glycoproteins from any IBV strain to be incorporated into the seasonal vaccine. We have characterized and ranked the growth profiles of the 71 IBV strains and the best performing strains were used for co-infection of eggs, followed by serial passaging to select for high-growth reassortant viruses. After serial passaging, we selected 10 clonal isolates based on their growth profiles assessed by hemagglutination and plaque-forming units. We then generated reverse genetics systems for the three clones that performed best in growth curves. The selected backbones were then used to generate different reassortant viruses with HA/NA combinations from high and low titer yielding wild type IBV. When the growth profiles of the recombinant reassortant viruses were tested, the low titer yielding HA/NA viruses with the selected backbones yielded higher titers similar to those from high titer yielding HA/NA combinations. The use of these IBV backbones with improved replication in eggs might increase yields for the influenza B virus components of seasonal influenza virus vaccines.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:58:31.398.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeffrey Johnson
SpeciesList	scientific name: Influenza C virus; NCBI TaxID: NCBITaxon:11552; scientific name: Influenza A virus (A/Florida/2e/2006(H3N2)); NCBI TaxID: 747679; scientific name: Influenza C virus (C/Great Lakes/1167/1954); NCBI TaxID: 11557; scientific name: Influenza B virus (B/Hawaii/10/2001); NCBI TaxID: 206189;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-25 13:15:59	ID requested
1	2023-03-10 13:01:07	announced
⏵ 2	2023-11-14 07:58:32	announced	2023-11-14: Updated project metadata.

Aslam S, Rajendran M, Kriti D, Kurland A, Johnson J, van Bakel H, Krammer F, Garc, í, a-Sastre A, Ayllon J, Generation of a high yield vaccine backbone for influenza B virus in embryonated chicken eggs. NPJ Vaccines, 8(1):12(2023) [pubmed]

submitter keyword: backbone, vaccine,Influenza B virus, high yield

Jeffrey R. Johnson
contact affiliation	Microbiology department at the Icahn School of Medicine atMount Sinai
contact email	jeffrey.johnson@mssm.edu
lab head
Jeffrey Johnson
contact affiliation	Icahn School of Medicine at Mount Sinai
contact email	jeffrey.johnson@mssm.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD036269

PRIDE project URI

• PRIDE
  1. PXD036269
     1. Label: PRIDE project
     2. Name: Generation of a high yield vaccine backbone for influenza B virus in embryonated chicken eggs