PXD036263

PXD036263 is an original dataset announced via ProteomeXchange.

Title	Expression of glycan receptors for influenza A viruses in mallards, chickens, and tufted ducks
Description	Influenza A virus (IAV) pandemics result from interspecies transmission events within the avian reservoir and further into mammals including humans. Investigating the molecular basis for virus–host interactions enabling this process is vital to understand zoonotic IAV spread. Receptor incompatibility has been suggested to limit zoonotic IAV transmission from the wild bird reservoir as well as between different bird species. Using glycoproteomics, we have studied the repertoires of expressed glycan structures with focus on putative receptors for IAV in mallards, chickens and tufted ducks; three bird species with different roles in the zoonotic ecology of IAV. The methodology used could not only pinpoint specific glycan structures to the specific glycosylation sites of identified glycoproteins but could also be used to successfully discriminate α2,3- from α2,6-linked terminal sialic acids by careful analysis of oxonium ions released from glycopeptides during MS/MS (MS2), and MS/MS/MS (MS3). Our analysis clearly demonstrated that all three bird species can produce complex α2,3 and α2,6-linked Neu5Ac N-glycans including α2,3-linked sialyl Lewis structures, as well as both N- and O- glycans terminated with both α2,3 and α2,6-linked Neu5Ac. Furthermore, we reveal many similarities in the repertoires of expressed receptors both between the bird species investigated and to previously published data from pigs and humans. Our findings of sialylated glycan structures previously anticipated to be mammalian specific in all three bird species have major implications for our understanding of the role of receptor incompatibility in interspecies transmission of IAV.
HostingRepository	PRIDE
AnnounceDate	2024-04-18
AnnouncementXML	Submission_2024-04-18_03:19:11.143.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ekaterina Mirgorodskaya
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-25 13:13:34	ID requested
⏵ 1	2024-04-18 03:19:12	announced

Nilsson J, Eriksson P, Naguib MM, Jax E, Sihlbom C, Olsson BM, Lundkvist Å, Olsen B, J, ä, rhult JD, Larson G, Ellstr, ö, m P, Expression of influenza A virus glycan receptor candidates in mallard, chicken, and tufted duck. Glycobiology, 34(3):(2024) [pubmed]

10.1093/glycob/cwad098;

submitter keyword: innate immunity,: Interspecies transmission, sialic acid receptor, tufted duck, glycoproteomics, mallard, influenza virus, adaptive viral mutations

Patrik Ellström
contact affiliation	Zoonosis Science Center, Department of Medical Sciences, Uppsala University, Uppsala SE-75185, Sweden
contact email	patrik.ellstrom@medsci.uu.se
lab head
Ekaterina Mirgorodskaya
contact affiliation	Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg
contact email	ekaterina.mirgorodskaya@gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD036263

PRIDE project URI

• PRIDE
  1. PXD036263
     1. Label: PRIDE project
     2. Name: Expression of glycan receptors for influenza A viruses in mallards, chickens, and tufted ducks