PXD036262

PXD036262 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling via MLF2-APEX2 fusion
Description	Proximity labeling approach to identify protein inside nuclear envelope blebs arising in Torsin-deficient HeLa cells. WT and TorsinKO cells were engineered to express MLF2-APEX2 fusion. APEX reaction was carried out via 1 mM H2O2 for 1 minute in the presence of biotin phenol. Biotinylated protein were captured via streptavidin beads. Each APEX reaction was accompanied by an untreated (no H2O2) control.
HostingRepository	PRIDE
AnnounceDate	2022-08-25
AnnouncementXML	Submission_2022-08-25_13:42:25.701.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036262
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sarah Prophet
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-25 13:05:33	ID requested
⏵ 1	2022-08-25 13:42:26	announced

Dataset with its publication pending

submitter keyword: MLF2, APEX, Torsin, nuclear envelope

Christian Schlieker
contact affiliation	Yale University, Department of Molecular Biophysics & Biochemistry
contact email	christian.schlieker@yale.edu
lab head
Sarah Prophet
contact affiliation	Yale University
contact email	sarah.prophet@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD036262

PRIDE project URI

• PRIDE
  1. PXD036262
     1. Label: PRIDE project
     2. Name: Proximity labeling via MLF2-APEX2 fusion