PXD036249

PXD036249 is an original dataset announced via ProteomeXchange.

Title	Negative N-terminal enrichment strategy for Mycobacterium tuberculosis H37Rv
Description	Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB), which resulted millions of deaths worldwide every year. Although the type strain M. tuberculosis H37Rv was sequenced in 1998, annotation errors of encoding genes have emerged in an endless stream. The annotation errors are particularly severe in the N-terminal and start sites of the genes. In this study, we applied a dimethylation labeling combined with negative enrichment strategy on M. tuberculosis H37Rv to characterize the N-terminal start sites of its annotated encoding genes. Totally, 18,285 peptides and 1,641 N-terminal peptides were identified from all the 2,728 database annotated proteins. The negative N-terminal enrichment strategy allowed the re-annotation of 12 genes’ N-terminal and identification of 6 novel genes in H37Rv. The comparative genomics approach could provide possible help for the re-annotation of 403 mis-annotated genes from Mycobacteriaceae. In addition, we verified the novel gene Rv3409, which was identified with 3 high-confident peptides, with the synthetic peptides and the expression of recombinant protein Rv3409. Altogether, our study and findings contributed to a better understanding of the N-terminal annotation of H37Rv, which will contribute to further biological studies of other species of Mycobacteriaceae in the future.
HostingRepository	iProX
AnnounceDate	2022-08-24
AnnouncementXML	Submission_2023-08-23_19:48:51.988.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yao Zhang
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	dimethylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-23 20:53:51	ID requested
⏵ 1	2023-08-23 19:48:52	announced

Dataset with its publication pending

submitter keyword: Mycobacterium tuberculosis, N-terminomic, Dimethylation, Negative enrichment

Ping Xu
contact affiliation	Beijing Proteome Research Center
contact email	xuping_bprc@126.com
lab head
Yao Zhang
contact affiliation	Beijing Proteome Research Center
contact email	zhangyaowsw@163.com
dataset submitter

iProX dataset URI