PXD036225 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Aedes aegypti Malpighian tubules are immunologically activated following systemic Toll activation |
Description | Canine heartworm is a widespread and potentially fatal mosquito-borne disease caused by infections with the parasitic nematode, Dirofilaria immitis. We have previously shown that systemic activation of the Toll immune pathway via silencing of the negative regulator Cactus in Aedes aegypti blocks parasite development in the Malpighian tubules, the mosquito renal organ. However, it was not established whether the Malpighian tubules were directly responding to Toll activation or were alternatively responding to upregulated proteins or other changes to the hemolymph driven by other tissues. Distinguishing these possibilities is crucial for developing more precise strategies to block D. immitis while potentially avoiding the fitness cost to the mosquito associated with Cactus silencing. This study defines the transcriptional response of Ae. aegypti Malpighian tubules after systemic Toll activation via intra-thoracic injection of dsCactus and found, like the response of whole mosquitoes, a significant increase in expression of Toll pathway target genes. Additionally, we identified a significant overlap between the transcriptional response of the Malpighian tubules and proteins upregulated in the hemolymph. Our data show that Malpighian tubules are capable of RNAi-mediated gene silencing and directly respond to dsCactus treatment by upregulating canonical Toll pathway targets. Though not definitive, the strong correspondence between the Malpighian tubule transcriptional and the hemolymph proteomic responses provides evidence that the tubules may contribute to mosquito humoral immunity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-08-27 |
AnnouncementXML | Submission_2024-08-27_03:43:16.375.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sutopa Dwivedi |
SpeciesList | scientific name: Aedes aegypti; NCBI TaxID: 7159; |
ModificationList | methylthiolated residue; acetylated residue; monohydroxylated residue; deamidated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-22 03:55:44 | ID requested | |
⏵ 1 | 2024-08-27 03:43:17 | announced | |
Publication List
10.1186/s13071-022-05567-2; |
Sneed SD, Dwivedi SB, DiGate C, Denecke S, Povelones M, Aedes aegypti Malpighian tubules are immunologically activated following systemic Toll activation. Parasit Vectors, 15(1):469(2022) [pubmed] |
Keyword List
submitter keyword: Aedes |
Hemolymph |
LFQ |
Cactus |
Toll |
Contact List
Michael Povelones |
contact affiliation | Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA |
contact email | mpove@upenn.edu |
lab head | |
Sutopa Dwivedi |
contact affiliation | School of Pathobiology, UPENN |
contact email | sutopabd@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036225
- Label: PRIDE project
- Name: Aedes aegypti Malpighian tubules are immunologically activated following systemic Toll activation