PXD036221

PXD036221 is an original dataset announced via ProteomeXchange.

Title	Pten regulates endocytic trafficking of cell adhesion and signaling molecules to pattern the retina
Description	The retina is an exquisitely patterned tissue, with neuronal somata positioned at regular intervals to completely sample the visual field. Cholinergic amacrine cells are spectacular exemplars of precision, distributing in two radial layers and tangentially, forming regular mosaics. Here, we investigated how the intracellular phosphatase Pten and the cell adhesion molecule Dscam cooperate to regulate amacrine cell patterning. Using double mutants to test epistasis, we found that Pten and Dscam function in parallel pathways to regulate amacrine cell positioning. Mechanistically, Pten regulates endocytic remodeling of cell adhesion molecules (Dscam, Megf10, Fat3), which are aberrantly redistributed in Pten conditional-knock-out (cKO) cholinergic amacrine cells. Furthermore, extracellular vesicle content, including that of cell adhesion and signaling molecules, is altered in Pten cKO retinas. Consequently, Wnt signalling is attenuated, the pharmacological disruption of which phenocopies Pten cKO amacrine cell patterning defects. Pten thus controls endocytic trafficking of critical cell adhesion/signaling molecules to control amacrine cell spacing.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:47:05.928.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD036221
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thomas Olender
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	(R)-5-oxo-1,4-tetrahydrothiazine-3-carboxylic acid; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-22 03:46:59	ID requested
⏵ 1	2024-10-07 13:47:06	announced

10.6019/PXD036221;

Touahri Y, Hanna J, Tachibana N, Okawa S, Liu H, David LA, Olender T, Vasan L, Pak A, Mehta DN, Chinchalongporn V, Balakrishnan A, Cantrup R, Dixit R, Mattar P, Saleh F, Ilnytskyy Y, Murshed M, Mains PE, Kovalchuk I, Lefebvre JL, Leong HS, Cayouette M, Wang C, Del Sol A, Brand M, Reese BE, Schuurmans C, Pten regulates endocytic trafficking of cell adhesion and Wnt signaling molecules to pattern the retina. Cell Rep, 43(4):114005(2024) [pubmed]

10.1016/j.celrep.2024.114005;

submitter keyword: cholinergic amacrine cell, cell adhesion molecules, mosaicism, extracellular vesicles,Retina, Wnt signaling, endocytic remodeling, Pten phosphatase, Dscam

Carol Schuurmans
contact affiliation	Senior scientist, Biological Sciences, Hurvitz Brain Sciences Research Program, Sunnybrook Research Institute, Toronto, Canada Professor, department of biochemistry, department of laboratory medicine and pathobiology, department of ophthalmology and vision sciences, University of Toronto, Canada Adjunct professor, department of biochemistry and molecular biology, University of Calgary, Canada
contact email	cschuurm@sri.utoronto.ca
lab head
Thomas Olender
contact affiliation	University of Ottawa & Ottawa Hospital Research Institute & Sprott Center for Stem Cell Research
contact email	tolender@toh.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD036221

PRIDE project URI

• PRIDE
  1. PXD036221
     1. Label: PRIDE project
     2. Name: Pten regulates endocytic trafficking of cell adhesion and signaling molecules to pattern the retina