PXD036201 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of phosphorylation sites of purified ORP8 from in vitro AMPK kinase assay |
Description | We investigated the potential role of AMPK in the phosphorylation of ORP8.In vitro kinase assay using purified ORP8 and kinase-active AMPK complex confirmed that ORP8 is the direct substrate of AMPK. Phosphorylated ORP8 from in vitro kinase assay was analyzed by HPLC-MS/MS. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:07:51.370.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | maomao pu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-08-21 04:39:21 | ID requested | |
1 | 2023-10-24 10:21:59 | announced | |
2 | 2023-11-14 09:06:29 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 06:07:52 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1093/procel/pwac063; |
Pu M, Zheng W, Zhang H, Wan W, Peng C, Chen X, Liu X, Xu Z, Zhou T, Sun Q, Neculai D, Liu W, ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover. Protein Cell, 14(9):653-667(2023) [pubmed] |
Keyword List
submitter keyword: AMPK,phosphorylation,ORP8 |
Contact List
Wei Liu |
contact affiliation | Department of Biochemistry and Department of Metabolic Medicine of the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China |
contact email | liuwei666@zju.edu.cn |
lab head | |
maomao pu |
contact affiliation | Zhejiang University |
contact email | maomaopu@zju.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD036201 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD036201
- Label: PRIDE project
- Name: Identification of phosphorylation sites of purified ORP8 from in vitro AMPK kinase assay