PXD036200

PXD036200 is an original dataset announced via ProteomeXchange.

Title	Identification of LC3-binding proteins on lipid droplets
Description	In the search for LD-localized proteins that may mediate lipophagy, we purified LDs from HeLa cells treated with oleic acid (OA), which stimulates LD biogenesis and lipophagy, together with chloroquine (CQ), which inhibits lysosomal degradation. We incubated the LD lysates with purified GST-LC3B, and then pulled down GST-LC3B for mass spectrometry analysis
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:56.291.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	maomao pu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-21 04:39:01	ID requested
1	2023-10-24 10:57:40	announced
⏵ 2	2023-11-14 09:07:01	announced	2023-11-14: Updated project metadata.

Pu M, Zheng W, Zhang H, Wan W, Peng C, Chen X, Liu X, Xu Z, Zhou T, Sun Q, Neculai D, Liu W, ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover. Protein Cell, 14(9):653-667(2023) [pubmed]

submitter keyword: lipid droplet,LC3

Wei Liu
contact affiliation	Department of Biochemistry and Department of Metabolic Medicine of the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China
contact email	liuwei666@zju.edu.cn
lab head
maomao pu
contact affiliation	Zhejiang University
contact email	maomaopu@zju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD036200

PRIDE project URI

• PRIDE
  1. PXD036200
     1. Label: PRIDE project
     2. Name: Identification of LC3-binding proteins on lipid droplets