PXD036198

PXD036198 is an original dataset announced via ProteomeXchange.

Title	Glycoproteomics revealed novel N-glycosylation biomarkers for early diagnosis of lung adenocarcinoma cancers
Description	Circulating biomarkers play important roles in diagnosis of malignant tumors. N-glycosylation is an important post-translation patter and obviously affect biological behaviors of malignant tumor cells. However, the role of N-glycosylation sites in early diagnosis of tumors still remains further investigation. In this study, plasma from 20 lung adenocarcinoma (LUAD), which were all classified as stage I, as well as 20 normal controls (NL) were labeled and screened by mass spectrometry (MS). Total 39 differential N-glycosylation sites were detected in LUAD, 17 were up-regulated and 22 were down-regulated. In all differential sites, ITGB3-680 showed highest potential in LUAD which showed 99.2% AUC, 95.0% SP and 95.0% SN. Besides, APOB-1523 (AUC: 89.0%, SP: 95.0%, SN: 70.0%), APOB-2982 (AUC: 86.8%, SP: 95.0%, SN: 45.0%) and LPAL2-101 (AUC: 81.1%, SP: 95.0%, SN: 47.4%) also acted as candidate biomarkers in LUAD. Combination analysis was then performed by random forest model, all samples were divided into training group (16 cases) and testing group (4 cases) and conducted by feature selection, machine learning, integrated model of classifier and model evaluation. And the results indicated that combination of differential sites could reach 100% AUC in both training and testing group. Taken together, our study revealed multiple N-glycosylation sites which could be applied as candidate biomarkers for early diagnosis diagnosis of LUAD.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:38:50.866.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhiqing jiang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-21 04:29:35	ID requested
1	2023-03-10 23:59:59	announced
⏵ 2	2023-11-14 08:38:53	announced	2023-11-14: Updated project metadata.

Fang K, Long Q, Liao Z, Zhang C, Jiang Z, Glycoproteomics revealed novel N-glycosylation biomarkers for early diagnosis of lung adenocarcinoma cancers. Clin Proteomics, 19(1):43(2022) [pubmed]

submitter keyword: lung adenocarcinoma

early diagnosis

circulating biomarkers

N-glycosylation

machine learning.

Jiang Zhiqiang
contact affiliation	The Second Affiliated Hospital of Chengdu Medical College (China National Nuclear Corporation 416 Hospital),
contact email	jiangzq_416@163.com
lab head
zhiqing jiang
contact affiliation	The second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation 416 Hospital
contact email	jiangzq_416@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD036198
     1. Label: PRIDE project
     2. Name: Glycoproteomics revealed novel N-glycosylation biomarkers for early diagnosis of lung adenocarcinoma cancers