PXD036193

PXD036193 is an original dataset announced via ProteomeXchange.

Title	Temporal proteomic characterization of COVID-19 infected mouse lungs
Description	We performed time-course proteomic profiling of lung tissues obtained from K18-hACE2 transgenic mice with TMT-based quantification using LC-MS/MS. The Proteome Discoverer analysis identified 10,844 protein groups, 8,279 of which were quantifiable. As a result of the comparison between our proteome data and the previously reported proteomes of COVID-19 infected lung tissues generated using LC-MS/MS analysis, we demonstrated the high comprehensiveness of our temporal proteome of COVID-19 infected lung tissues. To assess the correlation between our proteome and the proteins involved in COVID-19 pathophysiology, we examined the cellular processes of our proteome by performing the enrichment analysis of Gene Ontology biological processes (GOBP) and ConsensusPathDB software. In addition, we detailed temporal transitions of perturbed molecular networks activated along with COVID-19 infection by conducting clustering analysis that identifies early, middle, and late-responsive proteins. Also, we identified two key pathways, neutrophil extracellular trap formation (NETosis) and ciliogenesis, which can induce the severity-associated transition of the disease.
HostingRepository	PRIDE
AnnounceDate	2026-07-03
AnnouncementXML	Submission_2026-07-03_06:37:25.562.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dongyoon Shin
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-21 03:58:45	ID requested
⏵ 1	2026-07-03 06:37:26	announced

10.1016/J.MCPRO.2026.101618;

submitter keyword: COVID-19, tissue, proteomics, Tandem mass tag, LC-MS/MS

Youngsoo Kim
contact affiliation	Deparment of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea1Deparment of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea
contact email	biolab@snu.ac.kr
lab head
Dongyoon Shin
contact affiliation	Seoul National University
contact email	sdymath777@naver.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD036193

PRIDE project URI

• PRIDE
  1. PXD036193
     1. Label: PRIDE project
     2. Name: Temporal proteomic characterization of COVID-19 infected mouse lungs